Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101000 ptger4 sp1

ECD Proteins

Human PTGER4 Protein, hFc Tag

SKU:  PME101000     Target:  PTGER4     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100999 ptger2 sp1

ECD Proteins

Human PTGER2 Protein, hFc Tag

SKU:  PME100999     Target:  PTGER2     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100981 mmp2 sp1

ECD Proteins

Human MMP2 Protein, His Tag

SKU:  PME100981     Target:  MMP2     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100980 mmp11 sp1

ECD Proteins

Human MMP11 Protein, hFc Tag

SKU:  PME100980     Target:  MMP11     Tag:  C-Human Fc Tag

Price: 10ug $246; 50ug $960, 100ug $1440

sp-PME100977 MERTK hFc PAGE 300

ECD Proteins

Human MERTK Protein, hFc Tag

SKU:  PME100977     Target:  MERTK     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100580 app sp1

ECD Proteins

Human APP Protein, His Tag

SKU:  PME100580     Target:  APP     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100970 kras sp1

ECD Proteins

Human KRAS Protein, hFc Tag

SKU:  PME100970     Target:  KRAS     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-PME100967 ITGB5 Fig.1 SDS PAGE 1

ECD Proteins

Human ITGB5 Protein, hFc Tag

SKU:  PME100967     Target:  ITGB5     Tag:  C-Human Fc tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-pme100962 ifngr2 sp1

ECD Proteins

Human IFNGR2 Protein, hFc Tag

SKU:  PME100962     Target:  IFNGR2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100959 ifnar2 sp1

ECD Proteins

Human IFNAR2 Protein, hFc Tag

SKU:  PME100959     Target:  IFNAR2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100958 ifnar1 sp1

ECD Proteins

Human IFNAR1 Protein, His Tag

SKU:  PME100958     Target:  IFNAR1     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100952 lgals1 sp1

ECD Proteins

Human LGALS1 Protein, hFc Tag

SKU:  PME100952     Target:  LGALS1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100945 fn1 sp1

ECD Proteins

Human FN1 Protein, His Tag

SKU:  PME100945     Target:  FN1     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100941 egf sp1

ECD Proteins

Human EGF Protein, hFc Tag

SKU:  PME100941     Target:  EGF     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100250 folr2 sp1

ECD Proteins

Human FOLR2 Protein, mFc Tag

SKU:  PME100250     Target:  FOLR2     Tag:  C-mouse Fc Tag

Price: 10μg $91.00; 50μg $328.00 ; 100μg $488.00

sp-pme100937 cxcr2 sp1

ECD Proteins

Human CXCR2 Protein, hFc Tag

SKU:  PME100937     Target:  CXCR2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100936 cxcl9 sp1

ECD Proteins

Human CXCL9 Protein, hFc Tag

SKU:  PME100936     Target:  CXCL9     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-PME100935 CXCL12 Fig.1 SDS PAGE 1

ECD Proteins

Human CXCL12 Protein, hFc Tag

SKU:  PME100935     Target:  CXCL12     Tag:  N-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100934 cxcl1 sp1

ECD Proteins

Human CXCL1 Protein, hFc Tag

SKU:  PME100934     Target:  CXCL1     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-PME100933 CX3CR1 Fig.1 SDS PAGE 1

ECD Proteins

Human CX3CR1 Protein, hFc Tag

SKU:  PME100933     Target:  CX3CR1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme100930 gm csfr sp1

ECD Proteins

Human GM-CSFR Protein, hFc Tag

SKU:  PME100930     Target:  GM-CSFR     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100923 ccr5 sp1

ECD Proteins

Human CCR5 Protein, hFc Tag

SKU:  PME100923     Target:  CCR5     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100922 ccr2 sp1

ECD Proteins

Human CCR2 Protein, hFc Tag

SKU:  PME100922     Target:  CCR2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100921 ccr1 sp1

ECD Proteins

Human CCR1 Protein, hFc Tag

SKU:  PME100921     Target:  CCR1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100560 gprc5d sp1

ECD Proteins

Mouse GPRC5D Protein, hFc Tag

SKU:  PME100560     Target:  GPRC5D     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100919 ccl2 sp1

ECD Proteins

Human CCL2 Protein, hFc Tag

SKU:  PME100919     Target:  CCL2     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100917 cb2 sp1

ECD Proteins

Human CB2 Protein, hFc Tag

SKU:  PME100917     Target:  CB2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100916 calr sp1

ECD Proteins

Human CALR Protein, His Tag

SKU:  PME100916     Target:  CALR     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100915 brd4 sp1

ECD Proteins

Human BRD4 Protein, hFc Tag

SKU:  PME100915     Target:  BRD4     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100619 gas6 sp1

ECD Proteins

Human GAS6 Protein, His Tag

SKU:  PME100619     Target:  GAS6     Tag:  C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100128 adfam9 sp1

ECD Proteins

Human ADAM9 Protein, hFc Tag

SKU:  PME100128     Target:  ADAM9     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $450.00

sp-pme100895 magea4 sp1

ECD Proteins

Human MAGEA4 Protein, His Tag

SKU:  PME100895     Target:  MAGEA4     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00