Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100836 lrrc32 sp1

ECD Proteins

Human LRRC32 Protein, hFc Tag

SKU:  PME100836     Target:  LRRC32     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100835 cxcr5 sp1

ECD Proteins

Human CXCR5 Protein, hFc Tag

SKU:  PME100835     Target:  CXCR5     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100834 cxcr4 sp1

ECD Proteins

Human CXCR4 Protein, hFc Tag

SKU:  PME100834     Target:  CXCR4     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100833 cxcr3 sp1

ECD Proteins

Human CXCR3 Protein, hFc Tag

SKU:  PME100833     Target:  CXCR3     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme100832 clec2d sp1

ECD Proteins

Human CLEC2D Protein, hFc Tag

SKU:  PME100832     Target:  CLEC2D     Tag:  N-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100831 ceacam1 sp1

ECD Proteins

Human CEACAM1 Protein,His Tag

SKU:  PME100831     Target:  CEACAM1     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100830 c5ar1 sp1

ECD Proteins

Human C5AR1 Protein, hFc Tag

SKU:  PME100830     Target:  C5AR1     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100829 cd73 sp1

ECD Proteins

Human CD73 Protein, hFc Tag

SKU:  PME100829     Target:  CD73     Tag:  C-Human Fc Tag

Price: 10μg $137.00 ; 50μg $513.00 ; 100 μg $752.00

sp-pme100827 cd200r1 sp1

ECD Proteins

Human CD200R1 Protein, His Tag

SKU:  PME100827     Target:  CD200R1     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100826 ccr9 sp1

ECD Proteins

Human CCR9 Protein, hFc Tag

SKU:  PME100826     Target:  CCR9     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100825 ccr7 sp1

ECD Proteins

Human CCR7 Protein, hFc Tag

SKU:  PME100825     Target:  CCR7     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100824 ccr4 sp1

ECD Proteins

Human CCR4 (1-39) Protein, hFc Tag

SKU:  PME100824     Target:  CCR4     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100822 ceacam6 sp1

ECD Proteins

Human CEACAM6 Protein, His Tag

SKU:  PME100822     Target:  CEACAM6     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100821 mst1r sp1

ECD Proteins

Human MST1R Protein, hFc Tag

SKU:  PME100821     Target:  MST1R     Tag:  C-Human Fc Tag

Price: 10μg $137.00 ; 50μg $513.00 ; 100 μg $752.00

sp-pme100820 cxcr1 sp1

ECD Proteins

Human CXCR1 Protein, hFc Tag

SKU:  PME100820     Target:  CXCR1     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100818 cd39 sp1

ECD Proteins

Human CD39 Protein, hFc Tag

SKU:  PME100818     Target:  CD39     Tag:  C-Human Fc Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme100813 cd21 sp1

ECD Proteins

Human CD21 Protein, hFc Tag

SKU:  PME100813     Target:  CD21     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100812 cd147 sp1

ECD Proteins

Human CD147 Protein, hFc Tag

SKU:  PME100812     Target:  CD147     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100810 anpep sp1

ECD Proteins

Human ANPEP Protein, His Tag

SKU:  PME100810     Target:  ANPEP     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100809 il4ra sp1

ECD Proteins

Human IL4RA Protein, hFc Tag

SKU:  PME100809     Target:  IL4RA     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

products-pme

ECD Proteins

Human ITGAM Protein, hFc Tag

SKU:  PME100808     Target:  ITGAM     Tag:  C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-PME100806 SSTR2 hFc PAGE 300

ECD Proteins

Human SSTR2 Protein, hFc Tag

SKU:  PME100806     Target:  SSTR2     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100803 gpa33 sp1

ECD Proteins

Human GPA33 Protein, hFc Tag

SKU:  PME100803     Target:  GPA33     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100802 cdh3 sp1

ECD Proteins

Human CDH3 Protein, His Tag

SKU:  PME100802     Target:  CDH3     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100192 cd40 ligand sp1

ECD Proteins

Human CD40 Ligand Protein, hFc Tag

SKU:  PME100192     Target:  CD40 Ligand     Tag:  N-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100103 tgfbr1 sp1

ECD Proteins

Human TGFBR1 Protein, N-hFc Tag

SKU:  PME100103     Target:  TGFBR1     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100102 cd46 sp1

ECD Proteins

Human CD46 Protein, His Tag

SKU:  PME100102     Target:  CD46     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100099 egfr sp1

ECD Proteins

Human EGFR Protein, His Tag

SKU:  PME100099     Target:  EGFR     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100097 ror2 sp1

ECD Proteins

Human ROR2 Protein, His Tag

SKU:  PME100097     Target:  ROR2     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100096 clec12a sp1

ECD Proteins

Human CLEC12A Protein, His Tag

SKU:  PME100096     Target:  CLEC12A     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100095 her2 sp1

ECD Proteins

Human Her2 Protein, His Tag

SKU:  PME100095     Target:  Her2     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100094 taci sp1

ECD Proteins

Human TACI Protein, hFc Tag

SKU:  PME100094     Target:  TACI     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00