Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

Blog

Multi-Species Recombinant Proteins: The Secret Weapon in Antibody Drug Discovery

Explore how recombinant proteins derived from human, mouse, monkey, and dog enable more accurate antigen selection, cross-species validation, and improved...

16
Nov
Domain-Specific Protein Fragments in Therapeutic Antibody Discovery

Using multiple recombinant protein fragments from the same target—such as extracellular domains, functional regions, and membrane-proximal segments—has become an essential...

08
Dec

The List of ECD Proteins

sp-pme100556 il21 sp1

ECD Proteins

Human IL21 Protein, His Tag

SKU:  PME100556     Target:  IL21     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100886 il1ra sp1

ECD Proteins

Human IL1RA Protein, hFc Tag

SKU:  PME100886     Target:  IL1RA     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100885 il17b sp1

ECD Proteins

Human IL17B Protein, hFc Tag

SKU:  PME100885     Target:  IL17B     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100884 il17a sp1

ECD Proteins

Human IL17A Protein, hFc Tag

SKU:  PME100884     Target:  IL17A     Tag:  C-Human Fc Tag

Price: 10μg $91.00; 50μg $328.00 ; 100μg $488.00

sp-pme100555 il21 sp1

ECD Proteins

Human IL21 Protein, mFc Tag

SKU:  PME100555     Target:  IL21     Tag:  C-Mouse Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100882 il15 sp1

ECD Proteins

Human IL15 Protein, hFc Tag

SKU:  PME100882     Target:  IL15     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100881 il12rb1 sp1

ECD Proteins

Human IL12RB1 Protein, His Tag

SKU:  PME100881     Target:  IL12RB1     Tag:  C-6×His Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme100879 il10r sp1

ECD Proteins

Human IL10R Protein, hFc Tag

SKU:  PME100879     Target:  IL10R     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100878 il10 sp1

ECD Proteins

Human IL10 Protein, hFc Tag

SKU:  PME100878     Target:  IL10     Tag:  N-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100588 c mpl sp1

ECD Proteins

Human c-MPL Protein, hFc Tag

SKU:  PME100588     Target:  c-MPL     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100872 il13 sp1

ECD Proteins

Human IL13 Protein, hFc Tag

SKU:  PME100872     Target:  IL13     Tag:  C-Human Fc Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme100871 gnrhr sp1

ECD Proteins

Human GNRHR Protein, hFc Tag

SKU:  PME100871     Target:  GNRHR     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100870 gdnf sp1

ECD Proteins

Human GDNF Protein, hFc Tag

SKU:  PME100870     Target:  GDNF     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100867 fcmr sp1

ECD Proteins

Human FCMR Protein, hFc Tag

SKU:  PME100867     Target:  FCMR     Tag:  C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme100863 colec10 sp1

ECD Proteins

Human COLEC10 Protein, hFc Tag

SKU:  PME100863     Target:  COLEC10     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100862 cldn3 sp1

ECD Proteins

Human CLDN3 Protein, hFc Tag

SKU:  PME100862     Target:  CLDN3     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100861 itgav itgb1 sp1

ECD Proteins

Human ITGAV and ITGB1 Heterodimer Protein, His Tag and hFc Tag

SKU:  PME100861     Target:  ITGAV and ITGB1     Tag:  C-6×His Tag and C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100859 cd1a sp1

ECD Proteins

Human CD1A Protein, hFc Tag

SKU:  PME100859     Target:  CD1A     Tag:  C-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100μg $528.00

sp-pme100857 prom1 sp1

ECD Proteins

Human PROM1 Protein, hFc Tag

SKU:  PME100857     Target:  PROM1     Tag:  N-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100156 cb2 sp1

ECD Proteins

Human CB2 (1-33) Protein, hFc Tag

SKU:  PME100156     Target:  CB2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100855 vsig4 sp1

ECD Proteins

Human VSIG4 Protein, hFc Tag

SKU:  PME100855     Target:  VSIG4     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-PME100854 APRIL Fig.1 SDS PAGE 1

ECD Proteins

Human APRIL Protein, hFc Tag

SKU:  PME100854     Target:  APRIL     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme100853 tnfrsf11a sp1

ECD Proteins

Human TNFRSF11A Protein, hFc Tag

SKU:  PME100853     Target:  TNFRSF11A     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100852 ncr1 sp1

ECD Proteins

Human NCR1 Protein, hFc Tag

SKU:  PME100852     Target:  NCR1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100623 chi3l1 sp1

ECD Proteins

Human CHI3L1 Protein, His Tag

SKU:  PME100623     Target:  CHI3L1     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100604 clu sp1

ECD Proteins

Human CLU Protein, His Tag

SKU:  PME100604     Target:  CLU     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100848 cd99 sp1

ECD Proteins

Human CD99 Protein, hFc Tag

SKU:  PME100848     Target:  CD99     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100321 klrg1 sp1

ECD Proteins

Human KLRG1 Protein, hFc Tag

SKU:  PME100321     Target:  KLRG1     Tag:  N-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100844 kir2dl2 sp1

ECD Proteins

Human KIR2DL2 Protein, hFc Tag

SKU:  PME100844     Target:  KIR2DL2     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100841 il8 sp1

ECD Proteins

Human IL8 Protein, hFc Tag

SKU:  PME100841     Target:  IL8     Tag:  N-Human Fc tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100840 il7ra sp1

ECD Proteins

Human IL7RA Protein, hFc Tag

SKU:  PME100840     Target:  IL7RA     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100838 il1b sp1

ECD Proteins

Human IL1B Protein, hFc Tag

SKU:  PME100838     Target:  IL1B     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00