Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101711 acvr2b sp1

ECD Proteins

Human ACVR2B(36-105) Protein, mFc Tag

SKU:  PME101711     Target:  ACVR2B     Tag:  C-Mouse Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101710 acvr2b sp1

ECD Proteins

Human ACVR2B(18-41) Protein, mFc Tag

SKU:  PME101710     Target:  ACVR2B     Tag:  C-Mouse Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101709 acvr2a sp1

ECD Proteins

Human ACVR2A(101-135) Protein, mFc Tag

SKU:  PME101709     Target:  ACVR2A     Tag:  C-Mouse Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101700 il31ra sp1

ECD Proteins

Human IL31RA(20-225) Protein, mFc Tag

SKU:  PME101700     Target:  IL31RA     Tag:  C-Mouse Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101732 ighe sp1 1

ECD Proteins

Human IGHE(106-427) Protein, Flag and Strep Tag

SKU:  PME101732     Target:  IgHE     Tag:  C-Flag and Strep Tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101717 acvr2b sp1

ECD Proteins

Human ACVR2B(19-137) Protein, His Tag

SKU:  PME101717     Target:  ACVR2B     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101793 tgfb2 sp1

ECD Proteins

Human TGFB2(21-414) Protein, His Tag

SKU:  PME101793     Target:  TGFB2     Tag:  N-10×His tag

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101731 sortase a sp1

ECD Proteins

Staphylococcus aureus sortase A 5M Protein, His Tag

SKU:  PME101731     Target:  sortase A     Tag:  C-6×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101708 acvr2a sp1

ECD Proteins

Human ACVR2A(38-106) Protein, mFc Tag

SKU:  PME101708     Target:  ACVR2A     Tag:  C-Mouse Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101707 acvr2a sp1

ECD Proteins

Human ACVR2A(19-42) Protein, mFc Tag

SKU:  PME101707     Target:  ACVR2A     Tag:  C-Mouse Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101706 pd l1 sp1

ECD Proteins

Human PD-L1 Protein, Tag Free

SKU:  PME101706     Target:  PD-L1     Tag:  No tag

Price:10μg $96.00 ; 50μg $370.00 ; 100 μg $555.00

sp-pme101703 4 1bb sp1

ECD Proteins

Human 4-1BB(119-160) Protein, hFc Tag

SKU:  PME101703     Target:  4-1BB     Tag:  C-Human Fc tag

Price:10μg $96.00 ; 50μg $370.00 ; 100 μg $555.00

sp-pme101702 4 1bb sp1

ECD Proteins

Human 4-1BB(87-118) Protein, hFc Tag

SKU:  PME101702     Target:  4-1BB     Tag:  C-Human Fc tag

Price:10μg $96.00 ; 50μg $370.00 ; 100 μg $555.00

sp-pme101701 prame sp1

ECD Proteins

Human PRAME Protein, Flag Tag

SKU:  PME101701     Target:  PRAME     Tag:  C-3×Flag Tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101699 il31ra sp1

ECD Proteins

Human IL31RA(20-225) Protein, hFc Tag

SKU:  PME101699     Target:  IL31RA     Tag:  C-Human Fc tag

Price:10μg $75.00 ; 50μg $259.00 ; 100μg $389.00

sp-pme101698 qrfpr sp1

ECD Proteins

Human QRFPR Protein, hFc Tag

SKU:  PME101698     Target:  QRFPR     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101697 nk1r sp1

ECD Proteins

Human NK1R Protein, hFc Tag

SKU:  PME101697     Target:  NK1R     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101696 ca9 sp1

ECD Proteins

Human CA9(38-137) Protein, hFc Tag

SKU:  PME101696     Target:  CA9     Tag:  C-Human Fc tag

Price:10μg $78.00 ; 50μg $310.00 ; 100 μg $460.00

sp-pme101695 4 1bb sp1

ECD Proteins

Human 4-1BB(46-86) Protein, hFc Tag

SKU:  PME101695     Target:  4-1BB     Tag:  C-Human Fc tag

Price:10μg $96.00 ; 50μg $370.00 ; 100 μg $555.00

sp-pme101694 4 1bb sp1

ECD Proteins

Human 4-1BB(24-46) Protein, hFc Tag

SKU:  PME101694     Target:  4-1BB     Tag:  C-Human Fc tag

Price:10μg $96.00 ; 50μg $370.00 ; 100 μg $555.00

sp-pme101693 5t4 sp1

ECD Proteins

Human 5T4(256-355) Protein, hFc Tag

SKU:  PME101693     Target:  5T4     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101692 5t4 sp1

ECD Proteins

Human 5T4(233-255) Protein, hFc Tag

SKU:  PME101692     Target:  5T4     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101691 ca9 sp1

ECD Proteins

Human CA9(138-414) Protein, His Tag

SKU:  PME101691     Target:  CA9     Tag:  C-10×His tag

Price:10μg $78.00 ; 50μg $310.00 ; 100 μg $460.00

sp-pme101689 sema4a sp1

ECD Proteins

Human SEMA4A Protein, His Tag

SKU:  PME101689     Target:  SEMA4A     Tag:  C-10×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101688 enpp3 sp1

ECD Proteins

Human ENPP3(546-875) Protein, His Tag

SKU:  PME101688     Target:  ENPP3     Tag:  N-10×His tag

Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101687 enpp3 sp1

ECD Proteins

Human ENPP3(94-159) Protein, hFc Tag

SKU:  PME101687     Target:  ENPP3     Tag:  N-Human Fc tag

Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101686 5t4 sp1

ECD Proteins

Human 5T4(205-232) Protein, hFc Tag

SKU:  PME101686     Target:  5T4     Tag:  C-Human Fc tag

Price:10μg $68.00 ; 50μg $259.00 ; 100 μg $389.00

sp-pme101685 5t4 sp1

ECD Proteins

Human 5T4(164-208) Protein, hFc Tag

SKU:  PME101685     Target:  5T4     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101684 5t4 sp1

ECD Proteins

Human 5T4(140-171) Protein, hFc Tag

SKU:  PME101684     Target:  5T4     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101683 5t4 sp1

ECD Proteins

Human 5T4(114-140) Protein, hFc Tag

SKU:  PME101683     Target:  5T4     Tag:  C-Human Fc tag

Price:10μg $68.00 ; 50μg $259.00 ; 100 μg $389.00

sp-pme101682 5t4 sp1

ECD Proteins

Human 5T4(92-115) Protein, hFc Tag

SKU:  PME101682     Target:  5T4     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101681 5t4 sp1

ECD Proteins

Human 5T4(32-91) Protein, hFc Tag

SKU:  PME101681     Target:  5T4     Tag:  C-Human Fc tag

Price:10μg $68.00 ; 50μg $259.00 ; 100 μg $389.00