Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101767 cblb sp1

ECD Proteins

Human CBLB Protein, Flag Tag

SKU:  PME101767     Target:  CBLB     Tag:  C-3×Flag Tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101766 netrin 1 sp1

ECD Proteins

Human Netrin-1 Protein, Flag Tag

SKU:  PME101766     Target:  Netrin-1     Tag:  C-3×Flag Tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101764 procr sp1

ECD Proteins

Human PROCR Protein, hFc Tag

SKU:  PME101764     Target:  PROCR     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101763 nrg4 sp1

ECD Proteins

Human NRG4 Protein, hFc Tag

SKU:  PME101763     Target:  NRG4     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101762 hrh3 sp1

ECD Proteins

Human HRH3 Protein, hFc Tag

SKU:  PME101762     Target:  HRH3     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101761 cd58 sp1

ECD Proteins

Human CD58 Protein, His Tag

SKU:  PME101761     Target:  CD58     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101760 c1qbp sp1

ECD Proteins

Human C1QBP Protein, His Tag

SKU:  PME101760     Target:  C1QBP     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101759 fam171b sp1

ECD Proteins

Human FAM171B Protein, His Tag

SKU:  PME101759     Target:  FAM171B     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101758 erbb4 sp1

ECD Proteins

Human ERBB4 Protein, His Tag

SKU:  PME101758     Target:  ERBB4     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101756 lgr5 sp1

ECD Proteins

Human LGR5(22-561) Protein, hFc Tag

SKU:  PME101756     Target:  LGR5     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101755 nppa sp1

ECD Proteins

Human NPPA Protein, hFc Tag

SKU:  PME101755     Target:  NPPA     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101754 cd43 sp1

ECD Proteins

Human CD43 Protein, His Tag

SKU:  PME101754     Target:  CD43     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101753 scf sp1

ECD Proteins

Human SCF Protein, His Tag

SKU:  PME101753     Target:  SCF     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101752 epha10 sp1

ECD Proteins

Human EPHA10 Protein, His Tag

SKU:  PME101752     Target:  EPHA10     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101751 anxa2 sp1

ECD Proteins

Human ANXA2 Protein, His Tag

SKU:  PME101751     Target:  ANXA2     Tag:  N-10×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101750 timp2 sp1

ECD Proteins

Human TIMP2 Protein, His Tag

SKU:  PME101750     Target:  TIMP2     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101749 f7 sp1

ECD Proteins

Human F7 Protein, Flag Tag

SKU:  PME101749     Target:  F7     Tag:  C-3×Flag Tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101746 vegfc sp1

ECD Proteins

Human VEGFC Protein, hFc Tag

SKU:  PME101746     Target:  VEGFC     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101745 tmem119 sp1

ECD Proteins

Human TMEM119 Protein, hFc Tag

SKU:  PME101745     Target:  TMEM119     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101742 csf2rb sp1

ECD Proteins

Human CSF2RB Protein, His Tag

SKU:  PME101742     Target:  CSF2RB     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101741 ggt1 sp1

ECD Proteins

Human GGT1 Protein, His Tag

SKU:  PME101741     Target:  GGT1     Tag:  N-6×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101740 tnfrsf11b sp1

ECD Proteins

Human TNFRSF11B Protein, His Tag

SKU:  PME101740     Target:  TNFRSF11B     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101739 pecam1 sp1

ECD Proteins

Human PECAM1 Protein, His Tag

SKU:  PME101739     Target:  PECAM1     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101738 crp sp1

ECD Proteins

Human CRP Protein, His Tag

SKU:  PME101738     Target:  CRP     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101737 il18ra sp1

ECD Proteins

Human IL18RA Protein, His Tag

SKU:  PME101737     Target:  IL18RA     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101772 bai1 sp1

ECD Proteins

Human BAI1(31-926) Protein, Flag and Strep Tag

SKU:  PME101772     Target:  BAI1     Tag:  C-Flag and Strep Tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101765 reg4 sp1

ECD Proteins

Human REG4 Protein, hFc Tag

SKU:  PME101765     Target:  REG4     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00

sp-pme101748 f11 sp1

ECD Proteins

Human F11 Protein, Flag Tag

SKU:  PME101748     Target:  F11     Tag:  C-3×Flag Tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101744 sectm1 sp1

ECD Proteins

Human SECTM1 Protein, hFc Tag

SKU:  PME101744     Target:  SECTM1     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00

sp-pme101743 comp sp1

ECD Proteins

Human COMP Protein, His Tag

SKU:  PME101743     Target:  COMP     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00

sp-pme101757 lgr5 sp1 1

ECD Proteins

Human LGR5(447-561) Protein, hFc Tag

SKU:  PME101757     Target:  LGR5     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101747 cb1 sp1 1

ECD Proteins

Human CB1(1-116) Protein, mFc Tag

SKU:  PME101747     Target:  CB1     Tag:  C-Mouse Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00