Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101680 egfr sp1

ECD Proteins

Human EGFR(301-645) Protein, His Tag

SKU:  PME101680     Target:  EGFR     Tag:  C-10×His tag

Price:10μg $82.00 ; 50μg $320.00 ; 100 μg $480.00

sp-pme101679 ctsl sp1

ECD Proteins

Human CTSL Protein, His Tag

SKU:  PME101679     Target:  CTSL     Tag:  C-10×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101678 ctsd sp1

ECD Proteins

Human CTSD Protein, His Tag

SKU:  PME101678     Target:  CTSD     Tag:  C-10×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101676 baff sp1

ECD Proteins

Human BAFF Protein, mFc-His Tag

SKU:  PME101676     Target:  BAFF     Tag:  N-Mouse Fc tag and C-6×His tag

Price:10μg $96.00 ; 50μg $370.00 ; 100 μg $555.00

sp-pme101675 prlhr sp1

ECD Proteins

Human PRLHR Protein, hFc Tag

SKU:  PME101675     Target:  PRLHR     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101674 cdh6 sp1

ECD Proteins

Human CDH6(487-615) Protein, hFc Tag

SKU:  PME101674     Target:  CDH6     Tag:  C-Human Fc tag

Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101673 cdh6 sp1

ECD Proteins

Human CDH6(384-486) Protein, hFc Tag

SKU:  PME101673     Target:  CDH6     Tag:  C-Human Fc tag

Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101672 cdh6 sp1

ECD Proteins

Human CDH6(269-383) Protein, hFc Tag

SKU:  PME101672     Target:  CDH6     Tag:  C-Human Fc tag

Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101671 cdh6 sp1

ECD Proteins

Human CDH6(160-268) Protein, hFc Tag

SKU:  PME101671     Target:  CDH6     Tag:  C-Human Fc tag

Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101670 cdh6 sp1

ECD Proteins

Human CDH6(54-159) Protein, hFc Tag

SKU:  PME101670     Target:  CDH6     Tag:  C-Human Fc tag

Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101669 hgf sp1

ECD Proteins

Human HGF Protein, Flag Tag

SKU:  PME101669     Target:  HGF     Tag:  C-3×Flag Tag

Price:10μg $82.00 ; 50μg $320.00 ; 100 μg $480.00

sp-pme101668 il20rb sp1

ECD Proteins

Human IL20RB Protein, hFc Tag

SKU:  PME101668     Target:  IL20RB     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101667 egfr sp1

ECD Proteins

Human EGFR(25-389) Protein, His Tag

SKU:  PME101667     Target:  EGFR     Tag:  C-10×His tag

Price:10μg $82.00 ; 50μg $320.00 ; 100 μg $480.00

sp-pme101666 cd4 sp1

ECD Proteins

Human CD4 Protein, His Tag

SKU:  PME101666     Target:  CD4     Tag:  C-6×His tag

Price:10μg $72.00 ; 50μg $274.00 ; 100 μg $411.00

sp-pme101704 il11 sp1

ECD Proteins

Human IL11 Protein, Flag Tag

SKU:  PME101704     Target:  IL11     Tag:  C-Flag Tag

Price:10μg $82.00 ; 50μg $320.00 ; 100 μg $480.00

sp-pme m100124 light sp1

ECD Proteins

Mouse LIGHT Protein, hFc Tag

SKU:  PME-M100124     Target:  LIGHT     Tag:  N-Human Fc tag

Price: 10μg $85.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme m100123 dll3 sp1

ECD Proteins

Mouse DLL3(309-350) Protein, hFc Tag

SKU:  PME-M100123     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme m100122 gdf15 sp1

ECD Proteins

Mouse GDF15 Protein, hFc Tag

SKU:  PME-M100122     Target:  GDF15     Tag:  N-Human Fc tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme m100121 gdf15 sp1

ECD Proteins

Mouse GDF15 Protein, Flag Tag

SKU:  PME-M100121     Target:  GDF15     Tag:  C-3×Flag Tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme m100120 cd46 sp1

ECD Proteins

Mouse CD46 Protein, hFc Tag

SKU:  PME-M100120     Target:  CD46     Tag:  C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme c100084 sez6 sp1

ECD Proteins

Cynomolgus SEZ6 Protein, His Tag

SKU:  PME-C100084     Target:  SEZ6     Tag:  C-10×His tag

Price: 10μg $79.00 ; 50μg $281.00 ; 100μg $422.00

sp-pme c100083 light sp1

ECD Proteins

Cynomolgus LIGHT Protein, hFc Tag

SKU:  PME-C100083     Target:  LIGHT     Tag:  N-Human Fc tag

Price: 10μg $85.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme c100082 fcgr3a sp1

ECD Proteins

Cynomolgus FCGR3A Protein, hFc Tag

SKU:  PME-C100082     Target:  FCGR3A     Tag:  C-Human Fc tag

Price: 10μg $72.00 ; 50μg $274.00 ; 100μg $411.00

sp-pme c100081 tfrc sp1

ECD Proteins

Cynomolgus TFRC Protein, His Tag

SKU:  PME-C100081     Target:  TFRC     Tag:  N-10×His tag

Price: 10μg $79.00 ; 50μg $281.00 ; 100μg $422.00

sp-pme c100080 met sp1

ECD Proteins

Cynomolgus MET Protein, His Tag

SKU:  PME-C100080     Target:  MET     Tag:  C-10×His tag

Price: 10μg $79.00 ; 50μg $281.00 ; 100μg $422.00

sp-pme c100079 cd142 sp1

ECD Proteins

Cynomolgus CD142 Protein, His Tag

SKU:  PME-C100079     Target:  CD142     Tag:  C-10×His tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100μg $660.00

sp-pme101665 acvr1b sp1

ECD Proteins

Human ACVR1B Protein, hFc Tag

SKU:  PME101665     Target:  ACVR1B     Tag:  C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101664 epha3 sp1

ECD Proteins

Human EPHA3(436-541) Protein, hFc Tag

SKU:  PME101664     Target:  EPHA3     Tag:  C-Human Fc tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101662 epha3 sp1

ECD Proteins

Human EPHA3(21-324) Protein, His Tag

SKU:  PME101662     Target:  EPHA3     Tag:  C-10×His tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101661 dll3 sp1

ECD Proteins

Human DLL3(390-428) Protein, hFc Tag

SKU:  PME101661     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101660 dll3 sp1

ECD Proteins

Human DLL3(352-390) Protein, hFc Tag

SKU:  PME101660     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101659 dll3 sp1

ECD Proteins

Human DLL3(250-311) Protein, hFc Tag

SKU:  PME101659     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00