Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101739 pecam1 sp1

ECD Proteins

Human PECAM1 Protein, His Tag

SKU:  PME101739     Target:  PECAM1     Tag:  C-6×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101738 crp sp1

ECD Proteins

Human CRP Protein, His Tag

SKU:  PME101738     Target:  CRP     Tag:  C-6×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101737 il18ra sp1

ECD Proteins

Human IL18RA Protein, His Tag

SKU:  PME101737     Target:  IL18RA     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101772 bai1 sp1

ECD Proteins

Human BAI1(31-926) Protein, Flag and Strep Tag

SKU:  PME101772     Target:  BAI1     Tag:  C-Flag and Strep Tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101765 reg4 sp1

ECD Proteins

Human REG4 Protein, hFc Tag

SKU:  PME101765     Target:  REG4     Tag:  C-Human Fc tag

Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00

sp-pme101748 f11 sp1

ECD Proteins

Human F11 Protein, Flag Tag

SKU:  PME101748     Target:  F11     Tag:  C-3×Flag Tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101744 sectm1 sp1

ECD Proteins

Human SECTM1 Protein, hFc Tag

SKU:  PME101744     Target:  SECTM1     Tag:  C-Human Fc tag

Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00

sp-pme101743 comp sp1

ECD Proteins

Human COMP Protein, His Tag

SKU:  PME101743     Target:  COMP     Tag:  C-6×His tag

Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00

sp-pme101757 lgr5 sp1 1

ECD Proteins

Human LGR5(447-561) Protein, hFc Tag

SKU:  PME101757     Target:  LGR5     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101747 cb1 sp1 1

ECD Proteins

Human CB1(1-116) Protein, mFc Tag

SKU:  PME101747     Target:  CB1     Tag:  C-Mouse Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101736 lilra4 sp1 1

ECD Proteins

Human LILRA4 Protein, His Tag

SKU:  PME101736     Target:  LILRA4     Tag:  N-10×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101735 lilra4 sp1 1

ECD Proteins

Human LILRA4 Protein, His Tag

SKU:  PME101735     Target:  LILRA4     Tag:  C-10×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101734 ighe sp1 1

ECD Proteins

Human IGHE(106-427) Protein, His Tag

SKU:  PME101734     Target:  IgHE     Tag:  C-10×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101733 il33 sp1 1

ECD Proteins

Human IL33(109-270) Protein, His Tag

SKU:  PME101733     Target:  IL33     Tag:  N-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101730 sez6 sp1

ECD Proteins

Human SEZ6(833-925) Protein, hFc Tag

SKU:  PME101730     Target:  SEZ6     Tag:  C-Human Fc tag

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101729 sez6 sp1

ECD Proteins

Human SEZ6(768-835) Protein, hFc Tag

SKU:  PME101729     Target:  SEZ6     Tag:  C-Human Fc tag

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101728 sez6 sp1

ECD Proteins

Human SEZ6(705-768) Protein, hFc Tag

SKU:  PME101728     Target:  SEZ6     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101727 sez6 sp1

ECD Proteins

Human SEZ6(528-592) Protein, hFc Tag

SKU:  PME101727     Target:  SEZ6     Tag:  C-Human Fc tag

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101726 sez6 sp1

ECD Proteins

Human SEZ6(415-529) Protein, hFc Tag

SKU:  PME101726     Target:  SEZ6     Tag:  C-Human Fc tag

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101725 sez6 sp1

ECD Proteins

Human SEZ6(355-415) Protein, hFc Tag

SKU:  PME101725     Target:  SEZ6     Tag:  C-Human Fc tag

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101724 tnfsf15 sp1

ECD Proteins

Human TNFSF15 MTQ Protein, His Tag

SKU:  PME101724     Target:  TNFSF15     Tag:  N-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101723 tnfsf15 sp1

ECD Proteins

Human TNFSF15 Protein, His Tag

SKU:  PME101723     Target:  TNFSF15     Tag:  N-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101722 ghrhr sp1

ECD Proteins

Human GHRHR Protein, hFc Tag

SKU:  PME101722     Target:  GHRHR     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101721 drd2 sp1

ECD Proteins

Human DRD2 Protein, hFc Tag

SKU:  PME101721     Target:  DRD2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101720 neu1 sp1

ECD Proteins

Human NEU1 Protein, His Tag

SKU:  PME101720     Target:  NEU1     Tag:  C-10×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101719 lrp6 sp1

ECD Proteins

Human LRP6(20-1370) Protein, Flag Tag

SKU:  PME101719     Target:  LRP6     Tag:  C-3×Flag Tag

Price:10μg $91.00; 50μg $328.00 ; 100μg $488.00

sp-pme101718 nptx2 sp1

ECD Proteins

Human NPTX2 Protein, His Tag

SKU:  PME101718     Target:  NPTX2     Tag:  C-10×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101716 acvr2a sp1

ECD Proteins

Human ACVR2A(20-135) Protein, His Tag

SKU:  PME101716     Target:  ACVR2A     Tag:  N-10×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101715 tgfb3 sp1

ECD Proteins

Human TGFB3(301-412) Protein, hFc Tag

SKU:  PME101715     Target:  TGFB3     Tag:  N-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101714 oprk sp1

ECD Proteins

Human OPRK Protein, hFc Tag

SKU:  PME101714     Target:  OPRK     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101713 par1 sp1

ECD Proteins

Human PAR1 Protein, hFc Tag

SKU:  PME101713     Target:  PAR1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101712 acvr2b sp1

ECD Proteins

Human ACVR2B(98-134) Protein, mFc Tag

SKU:  PME101712     Target:  ACVR2B     Tag:  C-Mouse Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00