Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features For Immunization For Protein Activity Hot Druggable Targets

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

For Protein Activity

Human 4-1BB(46-86) Protein (PME101695)

Mouse 4-1BB Protein (PME-M100007)

Cynomolgus 4-1BB Protein (PME-C100023)

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

ECD Proteins

Human DLL3(216-273) Protein, hFc Tag

SKU: PME101658 Target: DLL3 Tag: C-Human Fc tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

ECD Proteins

Human FGFR2(313-363) Protein, hFc Tag

SKU: PME101657 Target: FGFR2 Tag: C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

ECD Proteins

Human FGFR2(313-363) Protein, mFc Tag

SKU: PME101656 Target: FGFR2 Tag: C-Mouse Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

ECD Proteins

Human GDF2 Protein, Flag Tag

SKU: PME101655 Target: GDF2 Tag: C-3×Flag Tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

ECD Proteins

Human MET(25-562) Protein, His Tag

SKU: PME101654 Target: MET Tag: C-10×His tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

ECD Proteins

Human CSF1R(105-202) Protein, hFc Tag

SKU: PME101652 Target: CSF1R Tag: C-Human Fc tag

Price: 10μg $78.00 ; 50μg $310.00 ; 100μg $460.00

ECD Proteins

Human ACVR2B(67-87) Protein, mFc Tag

SKU: PME101651 Target: ACVR2B Tag: C-Mouse Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

ECD Proteins

Human ACVR2A(68-88) Protein, mFc Tag

SKU: PME101650 Target: ACVR2A Tag: C-Mouse Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

ECD Proteins

Human TGFB1(30-390) Protein, His Tag

SKU: PME101649 Target: TGFB1 Tag: C-10×His tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

ECD Proteins

Human DLL3(311-352) Protein, hFc Tag

SKU: PME101648 Target: DLL3 Tag: C-Human Fc tag

Price: 10μg $82.00 ; 50μg $320.00 ; 100μg $480.00

ECD Proteins

Human GPA33(135-235) Protein, hFc Tag

SKU: PME101647 Target: GPA33 Tag: C-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

ECD Proteins

Human GPA33(22-139) Protein, hFc Tag

SKU: PME101646 Target: GPA33 Tag: C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

ECD Proteins

Human NRG1 Beta1(20-242) Protein, mFc Tag

SKU: PME101644 Target: NRG1 Beta1 Tag: C-Mouse Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

ECD Proteins

Human NRG1 Beta1(20-242) Protein, hFc Tag

SKU: PME101643 Target: NRG1 Beta1 Tag: C-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

ECD Proteins

Human MET(740-932) Protein, hFc Tag

SKU: PME101642 Target: MET Tag: C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

ECD Proteins

Human MET(516-656) Protein, hFc Tag

SKU: PME101641 Target: MET Tag: C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

ECD Proteins

Human TRPV1 Protein, hFc Tag

SKU: PME101185 Target: TRPV1 Tag: N-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

ECD Proteins

Human PTPRG Protein, His Tag

SKU: PME101130 Target: PTPRG Tag: C-6×His tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

ECD Proteins

Human PTPRG Protein, hFc Tag

SKU: PME101122 Target: PTPRG Tag: C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

ECD Proteins

Human USH2A Protein, N-hFc Tag

SKU: PME100702 Target: USH2A Tag: N-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

ECD Proteins

Human USH2A Protein, hFc Tag

SKU: PME100701 Target: USH2A Tag: C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00