Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

Blog

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The List of ECD Proteins

sp-pme101645 akt1 sp1

ECD Proteins

Human AKT1 Protein, Flag Tag

SKU:  PME101645     Target:  AKT1     Tag:  C-3×Flag Tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101636 ror2 sp1

ECD Proteins

Human ROR2(146-315) Protein, hFc Tag

SKU:  PME101636     Target:  ROR2     Tag:  C-Human Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101635 ceacam6 sp1

ECD Proteins

Human CEACAM6(233-319) Protein, hFc Tag

SKU:  PME101635     Target:  CEACAM6     Tag:  C-Human Fc tag

Price:10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme101634 ceacam6 sp1

ECD Proteins

Human CEACAM6(143-239) Protein, hFc Tag

SKU:  PME101634     Target:  CEACAM6     Tag:  C-Human Fc tag

Price:10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme101633 ceacam6 sp1

ECD Proteins

Human CEACAM6(35-144) Protein, hFc Tag

SKU:  PME101633     Target:  CEACAM6     Tag:  C-Human Fc tag

Price:10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme101624 fgf1 sp1

ECD Proteins

Human FGF1 Protein, hFc Tag

SKU:  PME101624     Target:  FGF1     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101623 cdh17 sp1

ECD Proteins

Human CDH17(668-787) Protein, hFc Tag

SKU:  PME101623     Target:  CDH17     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101622 cd117 sp1

ECD Proteins

Human CD117(411-524) Protein, hFc Tag

SKU:  PME101622     Target:  CD117     Tag:  C-Human Fc tag

Price:10μg $85.00; 50μg $330.00 ; 100 μg $500.00

elisa-PME100066 GPRC5D hFc His ELISA Fig2

ECD Proteins

Human GPRC5D Protein, hFc-His Tag

SKU:  PME100066     Target:  GPRC5D     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $99.00 ; 50μg $309.00 ; 100 μg $466.00

sp-PME100559 GPRC5D Fig.3 Elisa 2

ECD Proteins

Human GPRC5D Protein, mFc Tag

SKU:  PME100559     Target:  GPRC5D     Tag:  C-Mouse Fc Tag

Price: 10μg $99.00 ; 50μg $309.00 ; 100 μg $466.00

elisa-PME100677 GPRC5D hFc→DM91 ELISA Fig2

ECD Proteins

Human GPRC5D Protein, hFc Tag

SKU:  PME100677     Target:  GPRC5D     Tag:  C-Human Fc Tag

Price: 10μg $99.00 ; 50μg $309.00 ; 100 μg $466.00

sp-pme101621 cd117 sp1

ECD Proteins

Human CD117(309-412) Protein, hFc Tag

SKU:  PME101621     Target:  CD117     Tag:  C-Human Fc tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme101620 cd117 sp1

ECD Proteins

Human CD117(206-316) Protein, hFc Tag

SKU:  PME101620     Target:  CD117     Tag:  C-Human Fc tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme101619 b7 h7 sp1

ECD Proteins

Human B7-H7(223-344) Protein, hFc Tag

SKU:  PME101619     Target:  B7-H7     Tag:  C-Human Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101618 b7 h7 sp1

ECD Proteins

Human B7-H7(132-234) Protein, hFc Tag

SKU:  PME101618     Target:  B7-H7     Tag:  C-Human Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101617 b7 h7 sp1

ECD Proteins

Human B7-H7(23-137) Protein, hFc Tag

SKU:  PME101617     Target:  B7-H7     Tag:  C-Human Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101616 cdh3 sp1

ECD Proteins

Human CDH3(547-654) Protein, hFc Tag

SKU:  PME101616     Target:  CDH3     Tag:  C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101615 cdh3 sp1

ECD Proteins

Human CDH3(441-546) Protein, hFc Tag

SKU:  PME101615     Target:  CDH3     Tag:  C-Human Fc tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101614 cdh3 sp1

ECD Proteins

Human CDH3(329-440) Protein, hFc Tag

SKU:  PME101614     Target:  CDH3     Tag:  C-Human Fc tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101613 cdh3 sp1

ECD Proteins

Human CDH3(216-328) Protein, hFc Tag

SKU:  PME101613     Target:  CDH3     Tag:  C-Human Fc tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101612 fgf2 sp1

ECD Proteins

Human FGF2 Protein, Flag Tag

SKU:  PME101612     Target:  FGF2     Tag:  C-3×Flag Tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101611 ngf sp1

ECD Proteins

Human NGF(19-241) Protein, hFc Tag

SKU:  PME101611     Target:  NGF     Tag:  C-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101610 acvr2b sp1

ECD Proteins

Human ACVR2B Protein, mFc Tag

SKU:  PME101610     Target:  ACVR2B     Tag:  C-Mouse Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101609 acvr2a sp1

ECD Proteins

Human ACVR2A Protein, mFc Tag

SKU:  PME101609     Target:  ACVR2A     Tag:  C-Mouse Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101608 cdh17 sp1

ECD Proteins

Human CDH17(23-128) Protein, hFc Tag

SKU:  PME101608     Target:  CDH17     Tag:  C-Human Fc tag

Price: 10μg $68.00; 50μg $259.00 ; 100 μg $389.00

sp-pme101607 cdh1 sp1

ECD Proteins

Human CDH1(594-709) Protein, hFc Tag

SKU:  PME101607     Target:  CDH1     Tag:  C-Human Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101606 nectin 4 sp1

ECD Proteins

Human Nectin-4(238-349) Protein, hFc Tag

SKU:  PME101606     Target:  Nectin-4     Tag:  C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101605 nectin 4 sp1

ECD Proteins

Human Nectin-4(145-247) Protein, hFc Tag

SKU:  PME101605     Target:  Nectin-4     Tag:  C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101604 nectin 4 sp1

ECD Proteins

Human Nectin-4(32-147) Protein, hFc Tag

SKU:  PME101604     Target:  Nectin-4     Tag:  C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101603 cd117 sp1

ECD Proteins

Human CD117(113-211) Protein, hFc Tag

SKU:  PME101603     Target:  CD117     Tag:  C-Human Fc tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme101602 cd117 sp1

ECD Proteins

Human CD117(26-120) Protein, hFc Tag

SKU:  PME101602     Target:  CD117     Tag:  C-Human Fc tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme c100078 slitrk6 sp1

ECD Proteins

Cynomolgus SLITRK6 Protein, His Tag

SKU:  PME-C100078     Target:  SLITRK6     Tag:  C-10×His tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00