Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme c100077 cdh3 sp1

ECD Proteins

Cynomolgus CDH3 Protein, His Tag

SKU:  PME-C100077     Target:  CDH3     Tag:  C-10×His tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme c100076 cdcp1 sp1

ECD Proteins

Cynomolgus CDCP1 Protein, His Tag

SKU:  PME-C100076     Target:  CDCP1     Tag:  C-10×His tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme c100075 liv 1 sp1

ECD Proteins

Cynomolgus LIV-1 Protein, hFc Tag

SKU:  PME-C100075     Target:  LIV-1     Tag:  C-Human Fc tag

Price: 10μg $137.00 ; 50μg $513.00 ; 100 μg $752.00

sp-pme c100074 her2 sp1

ECD Proteins

Cynomolgus Her2 Protein, His Tag

SKU:  PME-C100074     Target:  Her2     Tag:  C-10×His tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme c100073 gpc3 sp1

ECD Proteins

Cynomolgus GPC3(510-559) Protein, hFc Tag

SKU:  PME-C100073     Target:  GPC3     Tag:  N-Human Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme c100072 gpc3 sp1

ECD Proteins

Cynomolgus GPC3(24-552) Protein, hFc Tag

SKU:  PME-C100072     Target:  GPC3     Tag:  C-Human Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme c100071 gpc3 sp1

ECD Proteins

Cynomolgus GPC3(24-552) Protein, His Tag

SKU:  PME-C100071     Target:  GPC3     Tag:  C-10×His tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100119 slitrk6 sp1

ECD Proteins

Mouse SLITRK6 Protein, His Tag

SKU:  PME-M100119     Target:  SLITRK6     Tag:  C-10×His tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme m100118 cdh3 sp1

ECD Proteins

Mouse CDH3 Protein, His Tag

SKU:  PME-M100118     Target:  CDH3     Tag:  C-10×His tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme m100117 gpc3 sp1

ECD Proteins

Mouse GPC3(510-559) Protein, hFc Tag

SKU:  PME-M100117     Target:  GPC3     Tag:  N-Human Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100116 acvr2b sp1

ECD Proteins

Mouse ACVR2B Protein, hFc Tag

SKU:  PME-M100116     Target:  ACVR2B     Tag:  C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme m100115 acvr2a sp1

ECD Proteins

Mouse ACVR2A Protein, hFc Tag

SKU:  PME-M100115     Target:  ACVR2A     Tag:  C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101601 msp2n2 sp1

ECD Proteins

Human MSP2N2 Protein, His Tag

SKU:  PME101601     Target:  MSP2N2     Tag:  N-6×His tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101600 msp1d1 sp1

ECD Proteins

Human MSP1D1 Protein, His Tag

SKU:  PME101600     Target:  MSP1D1     Tag:  N-6×His tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101599 msp1d1 sp1

ECD Proteins

Human MSP1D1(del H5) Protein, His Tag

SKU:  PME101599     Target:  MSP1D1     Tag:  N-6×His tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101598 il31ra sp1

ECD Proteins

Human IL31RA Protein, mFc Tag

SKU:  PME101598     Target:  IL31RA     Tag:  C-Mouse Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101597 il31ra sp1

ECD Proteins

Human IL31RA Protein, hFc Tag

SKU:  PME101597     Target:  IL31RA     Tag:  C-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101596 cdh3 sp1

ECD Proteins

Human CDH3(108-215) Protein, hFc Tag

SKU:  PME101596     Target:  CDH3     Tag:  C-Human Fc tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101595 ror2 sp1

ECD Proteins

Human ROR2(304-403) Protein, hFc Tag

SKU:  PME101595     Target:  ROR2     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101594 ror2 sp1

ECD Proteins

Human ROR2(34-168) Protein, hFc Tag

SKU:  PME101594     Target:  ROR2     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101592 pd l1 sp1

ECD Proteins

Human PD-L1(128-238) Protein, hFc Tag

SKU:  PME101592     Target:  PD-L1     Tag:  C-Human Fc tag

Price: 10μg $96.00 ; 50μg $370.00 ; 100 μg $555.00

sp-pme101591 pd l1 sp1

ECD Proteins

Human PD-L1(19-132) Protein, hFc Tag

SKU:  PME101591     Target:  PD-L1     Tag:  C-Human Fc tag

Price: 10μg $96.00 ; 50μg $370.00 ; 100 μg $555.00

sp-pme101590 b7 h3 sp1

ECD Proteins

Human B7-H3(140-248) Protein, hFc Tag

SKU:  PME101590     Target:  B7-H3     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101589 b7 h3 sp1

ECD Proteins

Human B7-H3(29-144) Protein, hFc Tag

SKU:  PME101589     Target:  B7-H3     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101588 itpripl1 sp1

ECD Proteins

Human ITPRIPL1 Protein, hFc Tag

SKU:  PME101588     Target:  ITPRIPL1     Tag:  C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101587 axl sp1

ECD Proteins

Human AXL(332-451) Protein, hFc Tag

SKU:  PME101587     Target:  AXL     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101586 axl sp1

ECD Proteins

Human AXL(223-335) Protein, hFc Tag

SKU:  PME101586     Target:  AXL     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101585 axl sp1

ECD Proteins

Human AXL(129-226) Protein, hFc Tag

SKU:  PME101585     Target:  AXL     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101584 axl sp1

ECD Proteins

Human AXL(27-138) Protein, hFc Tag

SKU:  PME101584     Target:  AXL     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101583 axl sp1

ECD Proteins

Human AXL(26-92) Protein, hFc Tag

SKU:  PME101583     Target:  AXL     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101582 ror1 sp1

ECD Proteins

Human ROR1(148-311) Protein, hFc Tag

SKU:  PME101582     Target:  ROR1     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101581 ror1 sp1

ECD Proteins

Human ROR1(30-164) Protein, hFc Tag

SKU:  PME101581     Target:  ROR1     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00