Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101516 ifngr1 sp1 1

ECD Proteins

Human IFNGR1 Protein, His Tag

SKU:  PME101516     Target:  IFNGR1     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101515 dpep3 sp1

ECD Proteins

Human DPEP3 Protein, His Tag

SKU:  PME101515     Target:  DPEP3     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101514 trhr sp1

ECD Proteins

Human TRHR Protein, hFc Tag

SKU:  PME101514     Target:  TRHR     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101513 or1a1 sp1

ECD Proteins

Human OR1A1 Protein, hFc Tag

SKU:  PME101513     Target:  OR1A1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101512 gpa33 sp1

ECD Proteins

Human GPA33 Protein, His Tag

SKU:  PME101512     Target:  GPA33     Tag:  C-10×His tag

Price:10μg $71.00 ; 50μg $259.00 ; 100μg $389.00

sp-pme101511 cd73 sp1

ECD Proteins

Human CD73 Protein, His Tag

SKU:  PME101511     Target:  CD73     Tag:  C-10×His tag

Price:10μg $137.00 ; 50μg $513.00 ; 100 μg $752.00

sp-pme101510 cd2 sp1

ECD Proteins

Human CD2 Protein, His Tag

SKU:  PME101510     Target:  CD2     Tag:  C-10×His tag

Price:10μg $71.00 ; 50μg $259.00 ; 100μg $389.00

sp-pme101508 osm sp1

ECD Proteins

Human OSM Protein, His Tag

SKU:  PME101508     Target:  OSM     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101507 il1r2 sp1

ECD Proteins

Human IL1R2 Protein, mFc Tag

SKU:  PME101507     Target:  IL1R2     Tag:  C-mouse Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101506 il1a sp1

ECD Proteins

Human IL1A Protein, mFc Tag

SKU:  PME101506     Target:  IL1A     Tag:  C-mouse Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101503 il1r2 sp1

ECD Proteins

Human IL1R2 Protein, hFc Tag

SKU:  PME101503     Target:  IL1R2     Tag:  C-Human Fc tag

Price:10μg $71.00 ; 50μg $259.00 ; 100μg $389.00

sp-pme101502 il1ra sp1

ECD Proteins

Human IL1RA Protein, His Tag

SKU:  PME101502     Target:  IL1RA     Tag:  C-10×His tag

Price:10μg $71.00 ; 50μg $259.00 ; 100μg $389.00

sp-pme101501 ca12 sp1

ECD Proteins

Human CA12 Protein, His Tag

SKU:  PME101501     Target:  CA12     Tag:  C-10×His tag

Price:10μg $71.00 ; 50μg $259.00 ; 100μg $389.00

sp-pme101497 ceacam5 sp1

ECD Proteins

Human CEACAM5(589-685) Protein, hFc Tag

SKU:  PME101497     Target:  CEACAM5     Tag:  C-Human Fc tag

Price:10μg $87.00 ; 50μg $318.00 ; 100μg $478.00

sp-pme101496 ceacam5 sp1

ECD Proteins

Human CEACAM5(496-685) Protein, His Tag

SKU:  PME101496     Target:  CEACAM5     Tag:  C-10×His tag

Price:10μg $87.00 ; 50μg $318.00 ; 100μg $478.00

sp-pme101489 nkg2c sp1

ECD Proteins

Human NKG2C Protein, hFc Tag

SKU:  PME101489     Target:  NKG2C     Tag:  N-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101488 igf2 sp1

ECD Proteins

Human IGF2 Protein, hFc Tag

SKU:  PME101488     Target:  IGF2     Tag:  N-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101487 ccl7 sp1

ECD Proteins

Human CCL7 Protein, hFc Tag

SKU:  PME101487     Target:  CCL7     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101486 adam17 sp1

ECD Proteins

Human ADAM17 Protein, hFc Tag

SKU:  PME101486     Target:  ADAM17     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101485 il29 sp1

ECD Proteins

Human IL29 Protein, hFc Tag

SKU:  PME101485     Target:  IL29     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101484 il7 sp1

ECD Proteins

Human IL7 Protein, hFc Tag

SKU:  PME101484     Target:  IL7     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101483 lair2 sp1

ECD Proteins

Human LAIR2 Protein, hFc Tag

SKU:  PME101483     Target:  LAIR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101482 he4 sp1

ECD Proteins

Human HE4 Protein, hFc Tag

SKU:  PME101482     Target:  HE4     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101481 efna4 sp1

ECD Proteins

Human EFNA4 Protein, hFc Tag

SKU:  PME101481     Target:  EFNA4     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101480 dkk3 sp1

ECD Proteins

Human DKK3 Protein, hFc Tag

SKU:  PME101480     Target:  DKK3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101479 efna1 sp1

ECD Proteins

Human EFNA1 Protein, hFc Tag

SKU:  PME101479     Target:  EFNA1     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101478 cd105 sp1

ECD Proteins

Human CD105 Protein, His Tag

SKU:  PME101478     Target:  CD105     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101477 ccl22 sp1

ECD Proteins

Human CCL22 Protein, hFc Tag

SKU:  PME101477     Target:  CCL22     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101467 itgav itgb5 sp1

ECD Proteins

Human ITGAV and ITGB5 Heterodimer Protein, His Tag and hFc Tag

SKU:  PME101467     Target:  ITGAV and ITGB5     Tag:  C-6×His tag and C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101546 crtam sp1

ECD Proteins

Human CRTAM Protein, hFc Tag

SKU:  PME101546     Target:  CRTAM     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101520 pth1r sp1 1

ECD Proteins

Human PTH1R Protein, hFc Tag

SKU:  PME101520     Target:  PTH1R     Tag:  C-Human Fc tag

Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00

sp-pme101509 b4galt1 sp1

ECD Proteins

Human B4GALT1 Protein, His Tag

SKU:  PME101509     Target:  B4GALT1     Tag:  N-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00