Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme c100067 crtam sp1

ECD Proteins

Cynomolgus CRTAM Protein, His Tag

SKU:  PME-C100067     Target:  CRTAM     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101551 acvr2b sp1

ECD Proteins

Human ACVR2B Protein, hFc Tag

SKU:  PME101551     Target:  ACVR2B     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101550 acvr2a sp1

ECD Proteins

Human ACVR2A Protein, hFc Tag

SKU:  PME101550     Target:  ACVR2A     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101549 apoe2 sp1

ECD Proteins

Human APOE2 Protein, His Tag

SKU:  PME101549     Target:  APOE2     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101548 apoe4 sp1

ECD Proteins

Human APOE4 Protein, His Tag

SKU:  PME101548     Target:  APOE4     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101547 crtam sp1

ECD Proteins

Human CRTAM Protein, mFc Tag

SKU:  PME101547     Target:  CRTAM     Tag:  C-mouse Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101545 cadm1 sp1

ECD Proteins

Human CADM1 Protein, mFc Tag

SKU:  PME101545     Target:  CADM1     Tag:  C-mouse Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101544 cadm1 sp1

ECD Proteins

Human CADM1 Protein, hFc Tag

SKU:  PME101544     Target:  CADM1     Tag:  C-Human Fc tag

Price:10μg $79.00 ; 50μg $281.00 ; 100μg $422.00

sp-pme101543 mmp9 sp1

ECD Proteins

Human MMP9(32-488) Protein, hFc Tag

SKU:  PME101543     Target:  MMP9     Tag:  C-Human Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101542 dll1 sp1

ECD Proteins

Human DLL1 Protein, His Tag

SKU:  PME101542     Target:  DLL1     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101541 kir2dl1 sp1

ECD Proteins

Human KIR2DL1 Protein, His Tag

SKU:  PME101541     Target:  KIR2DL1     Tag:  C-10×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101540 clec1a sp1

ECD Proteins

Human CLEC1A Protein, His Tag

SKU:  PME101540     Target:  CLEC1A     Tag:  C-10×His tag

Price:10μg $75.00 ; 50μg $274.00 ; 100μg $411.00

sp-pme101539 ly6e sp1

ECD Proteins

Human LY6E Protein, hFc Tag

SKU:  PME101539     Target:  LY6E     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101538 fgf19 sp1

ECD Proteins

Human FGF19 Protein, hFc Tag

SKU:  PME101538     Target:  FGF19     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101537 tnfsf15 sp1

ECD Proteins

Human TNFSF15 Protein, His Tag

SKU:  PME101537     Target:  TNFSF15     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101536 alpl sp1

ECD Proteins

Human ALPL Protein, His Tag

SKU:  PME101536     Target:  ALPL     Tag:  C-10×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101535 cd3e sp1

ECD Proteins

Human CD3E Protein, His Tag

SKU:  PME101535     Target:  CD3E     Tag:  C-10×His tag

Price:10μg $75.00 ; 50μg $274.00 ; 100μg $411.00

sp-pme101534 znrf3 sp1

ECD Proteins

Human ZNRF3 Protein, hFc Tag

SKU:  PME101534     Target:  ZNRF3     Tag:  N-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101533 adamts13 sp1

ECD Proteins

Human ADAMTS13 Protein, Flag Tag

SKU:  PME101533     Target:  ADAMTS13     Tag:  C-3×Flag Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101531 alpi sp1

ECD Proteins

Human ALPI Protein, His Tag

SKU:  PME101531     Target:  ALPI     Tag:  C-10×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101530 steap1 sp1

ECD Proteins

Human STEAP1 Protein, hFc Tag

SKU:  PME101530     Target:  STEAP1     Tag:  N-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101529 fcrl1 sp1

ECD Proteins

Human FCRL1 Protein, hFc Tag

SKU:  PME101529     Target:  FCRL1     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101527 ifnar2 sp1

ECD Proteins

Human IFNAR2 Protein, His Tag

SKU:  PME101527     Target:  IFNAR2     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101526 sparc sp1

ECD Proteins

Human SPARC Protein, His Tag

SKU:  PME101526     Target:  SPARC     Tag:  C-10×His tag

Price:10μg $71.00 ; 50μg $259.00 ; 100μg $389.00

sp-pme101525 ednrb sp1

ECD Proteins

Human EDNRB Protein, hFc Tag

SKU:  PME101525     Target:  EDNRB     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101524 cfd sp1

ECD Proteins

Human CFD Protein, His Tag

SKU:  PME101524     Target:  CFD     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101523 tshr sp1

ECD Proteins

Human TSHR(23-410) Protein, hFc Tag

SKU:  PME101523     Target:  TSHR     Tag:  N-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101522 cd3g sp1

ECD Proteins

Human CD3G Protein, hFc Tag

SKU:  PME101522     Target:  CD3G     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101521 vegfd sp1 1

ECD Proteins

Human VEGFD Protein, hFc Tag

SKU:  PME101521     Target:  VEGFD     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101519 gpbar1 sp1 1

ECD Proteins

Human GPBAR1 Protein, hFc Tag

SKU:  PME101519     Target:  GPBAR1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101518 slc44a4 sp1 1

ECD Proteins

Human SLC44A4 Protein, hFc Tag

SKU:  PME101518     Target:  SLC44A4     Tag:  N-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101517 lamp5 sp1 1

ECD Proteins

Human LAMP5 Protein, His Tag

SKU:  PME101517     Target:  LAMP5     Tag:  C-10×His tag

Price:10μg $71.00 ; 50μg $259.00 ; 100μg $389.00