Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101580 il36g sp1

ECD Proteins

Human IL36G Protein, hFc Tag

SKU:  PME101580     Target:  IL36G     Tag:  C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101579 il36a sp1

ECD Proteins

Human IL36A Protein, hFc Tag

SKU:  PME101579     Target:  IL36A     Tag:  C-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101578 dll3 sp1

ECD Proteins

Human DLL3(27-175) Protein, N-MBP Tag and C-10×His tag

SKU:  PME101578     Target:  DLL3     Tag:  N-MBP tag and C-10×His tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101577 b7 h4 sp1

ECD Proteins

Human B7-H4(147-259) Protein, hFc Tag

SKU:  PME101577     Target:  B7-H4     Tag:  C-Human Fc tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101576 b7 h4 sp1

ECD Proteins

Human B7-H4(25-152) Protein, hFc Tag

SKU:  PME101576     Target:  B7-H4     Tag:  C-Human Fc tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101575 ceacam5 sp1

ECD Proteins

Human CEACAM5(496-592) Protein, hFc Tag

SKU:  PME101575     Target:  CEACAM5     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101574 ceacam5 sp1

ECD Proteins

Human CEACAM5(411-500) Protein, hFc Tag

SKU:  PME101574     Target:  CEACAM5     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101573 ceacam5 sp1

ECD Proteins

Human CEACAM5(316-417) Protein, hFc Tag

SKU:  PME101573     Target:  CEACAM5     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101572 ceacam5 sp1

ECD Proteins

Human CEACAM5(233-322) Protein, hFc Tag

SKU:  PME101572     Target:  CEACAM5     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101571 ceacam5 sp1

ECD Proteins

Human CEACAM5(145-239) Protein, hFc Tag

SKU:  PME101571     Target:  CEACAM5     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101570 ceacam5 sp1

ECD Proteins

Human CEACAM5(35-144) Protein, hFc Tag

SKU:  PME101570     Target:  CEACAM5     Tag:  C-Human Fc tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101568 fcgr1a sp1

ECD Proteins

Human FCGR1A Protein, His Tag

SKU:  PME101568     Target:  FCGR1A     Tag:  C-6×His tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101561 g csf sp1

ECD Proteins

Human G-CSF Protein, hFc Tag

SKU:  PME101561     Target:  G-CSF     Tag:  C-Human Fc tag

Price: 10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101560 fcgr1a sp1

ECD Proteins

Human FCGR1A Protein, hFc Tag

SKU:  PME101560     Target:  FCGR1A     Tag:  C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101559 apoa1 sp1

ECD Proteins

Human APOA1 Protein, His Tag

SKU:  PME101559     Target:  APOA1     Tag:  C-10×His tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101558 cd19 sp1

ECD Proteins

Human CD19(M75V,R76S,F85S) Protein, hFc Tag

SKU:  PME101558     Target:  CD19     Tag:  C-Human Fc tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101557 cd19 sp1

ECD Proteins

Human CD19(M75I,L82V,F83L) Protein, hFc Tag

SKU:  PME101557     Target:  CD19     Tag:  C-Human Fc tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101556 clm5 sp1

ECD Proteins

Human CLM5 Protein, hFc Tag

SKU:  PME101556     Target:  CLM5     Tag:  C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101555 pvrig sp1

ECD Proteins

Human PVRIG(53-150) Protein, hFc Tag

SKU:  PME101555     Target:  PVRIG     Tag:  C-Human Fc tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101554 hsd17b10 sp1

ECD Proteins

Human HSD17B10 Protein, Flag Tag

SKU:  PME101554     Target:  HSD17B10     Tag:  C-3×Flag Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101553 AKT1 sp1

ECD Proteins

Human AKT1(409-480) Protein, hFc Tag

SKU:  PME101553     Target:  AKT1     Tag:  N-Human Fc tag

Price: 10μg $79.00 ; 50μg $281.00 ; 100μg $422.00

sp-pme101552 rnf133 sp1

ECD Proteins

Human RNF133 Protein, hFc Tag

SKU:  PME101552     Target:  RNF133     Tag:  C-Human Fc tag

Price: 10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

elisa-pme101593 il31 elisa2

ECD Proteins

Human IL31 Protein, His Tag

SKU:  PME101593     Target:  IL31     Tag:  C-10×His tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101567 cdh17 sp1

ECD Proteins

Human CDH17(668-777) Protein, hFc Tag

SKU:  PME101567     Target:  CDH17     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101566 cdh17 sp1

ECD Proteins

Human CDH17(450-566) Protein, hFc Tag

SKU:  PME101566     Target:  CDH17     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101565 cdh17 sp1

ECD Proteins

Human CDH17(341-449) Protein, hFc Tag

SKU:  PME101565     Target:  CDH17     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101564 cdh17 sp1

ECD Proteins

Human CDH17(245-340) Protein, hFc Tag

SKU:  PME101564     Target:  CDH17     Tag:  C-Human Fc tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101563 cdh17 sp1

ECD Proteins

Human CDH17(129-244) Protein, hFc Tag

SKU:  PME101563     Target:  CDH17     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101562 cdh17 sp1

ECD Proteins

Human CDH17(30-128) Protein, hFc Tag

SKU:  PME101562     Target:  CDH17     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme c100070 muc1 sp1

ECD Proteins

Cynomolgus MUC1(380-500) Protein, mFc Tag

SKU:  PME-C100070     Target:  MUC1     Tag:  C-Mouse Fc tag

Price:10μg $81.00 ; 50μg $296.00 ; 100μg $444.00

sp-pme c100069 muc1 sp1

ECD Proteins

Cynomolgus MUC1(380-500) Protein, hFc Tag

SKU:  PME-C100069     Target:  MUC1     Tag:  C-Human Fc tag

Price:10μg $81.00 ; 50μg $296.00 ; 100μg $444.00

sp-pme c100068 muc1 sp1

ECD Proteins

Cynomolgus MUC1(24-504) Protein, His Tag

SKU:  PME-C100068     Target:  MUC1     Tag:  C-10×His tag

Price:10μg $81.00 ; 50μg $296.00 ; 100μg $444.00