Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme c100066 dll3 sp1

ECD Proteins

Cynomolgus DLL3(350-488) Protein, hFc Tag

SKU:  PME-C100066     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme c100065 dll3 sp1

ECD Proteins

Cynomolgus DLL3(426-488) Protein, hFc Tag

SKU:  PME-C100065     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme c100064 dll3 sp1

ECD Proteins

Cynomolgus DLL3(388-488) Protein, hFc Tag

SKU:  PME-C100064     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme c100063 dll3 sp1 1

ECD Proteins

Cynomolgus DLL3(309-488) Protein, hFc Tag

SKU:  PME-C100063     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme c100062 dll3 sp1 1

ECD Proteins

Cynomolgus DLL3(272-488) Protein, His Tag

SKU:  PME-C100062     Target:  DLL3     Tag:  C-10×His tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme c100061 fcrl5 sp1 1

ECD Proteins

Cynomolgus FCRL5 Protein, His Tag

SKU:  PME-C100061     Target:  FCRL5     Tag:  C-10×His tag

Price: 10μg $100.00; 50μg $358.00 ; 100μg $528.00

sp-pme m100114 folr1 sp1

ECD Proteins

Mouse FOLR1 Protein, His Tag

SKU:  PME-M100114     Target:  FOLR1     Tag:  C-10×His tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme m100113 fcrl5 sp1

ECD Proteins

Mouse FCRL5 Protein, His Tag

SKU:  PME-M100113     Target:  FCRL5     Tag:  C-10×His tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101532 dll3 sp1 1

ECD Proteins

Human DLL3(176-215) Protein, hFc Tag

SKU:  PME101532     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101505 dll3 sp1 1

ECD Proteins

Human DLL3(428-492) Protein, hFc Tag

SKU:  PME101505     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101504 dll3 sp1 1

ECD Proteins

Human DLL3(311-492) Protein, hFc Tag

SKU:  PME101504     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101499 dll3 sp1 1

ECD Proteins

Human DLL3(390-492) Protein, hFc Tag

SKU:  PME101499     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101498 dll3 sp1

ECD Proteins

Human DLL3(352-492) Protein, hFc Tag

SKU:  PME101498     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101491 vegf165 sp1

ECD Proteins

Human VEGF165 Protein, hFc Tag

SKU:  PME101491     Target:  VEGF165     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100μg $480.00

sp-pme101490 vegf121 sp1 1

ECD Proteins

Human VEGF121 Protein, hFc Tag

SKU:  PME101490     Target:  VEGF121     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100μg $480.00

sp-pme100742 adamts13 sp1

ECD Proteins

Human ADAMTS13 Protein, His Tag

SKU:  PME100742     Target:  ADAMTS13     Tag:  C-6×His tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme f100002 il31ra sp1

ECD Proteins

Feline IL31RA Protein, mFc Tag

SKU:  PME-F100002     Target:  IL31RA     Tag:  C-Mouse Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme d100010 il31ra sp1

ECD Proteins

Canine IL31RA Protein, mFc Tag

SKU:  PME-D100010     Target:  IL31RA     Tag:  C-Mouse Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme c100060 bcma sp1

ECD Proteins

Cynomolgus BCMA Protein, hFc Tag

SKU:  PME-C100060     Target:  BCMA     Tag:  C-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme c100059 ror2 sp1

ECD Proteins

Cynomolgus ROR2 Protein, N-His Tag

SKU:  PME-C100059     Target:  ROR2     Tag:  N-10×His tag

Price: 10μg $112.00; 50μg $409.00 ; 100 μg $614.00

sp-pme c100058 ror2 sp1

ECD Proteins

Cynomolgus ROR2 Protein, C-His Tag

SKU:  PME-C100058     Target:  ROR2     Tag:  C-10×His tag

Price: 10μg $112.00; 50μg $409.00 ; 100 μg $614.00

sp-pme c100057 trop2 sp1

ECD Proteins

Cynomolgus Trop2 Protein, His Tag

SKU:  PME-C100057     Target:  Trop2     Tag:  C-10×His tag

Price: 10μg $104.00 ; 50μg $386.00 ; 100 μg $570.00

sp-pme c100026 muc1 sp1

ECD Proteins

Cynomolgus MUC1(254-373) Protein, His Tag

SKU:  PME-C100026     Target:  MUC1     Tag:  C-10×His tag

Price: 10μg $104.00 ; 50μg $386.00 ; 100 μg $570.00

sp-pme m100112 cdh6 sp1

ECD Proteins

Mouse CDH6 Protein, His Tag

SKU:  PME-M100112     Target:  CDH6     Tag:  C-10×His tag

Price: 10μg $104.00 ; 50μg $386.00 ; 100 μg $570.00

sp-pme101528 hgf sp1

ECD Proteins

Human HGF(1-728) Protein, hFc Tag

SKU:  PME101528     Target:  HGF     Tag:  C-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101500 il1ra sp1

ECD Proteins

Human IL1RA Protein, mFc Tag

SKU:  PME101500     Target:  IL1RA     Tag:  C-mouse Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme101475 folr3 sp1

ECD Proteins

Human FOLR3 Protein, His Tag

SKU:  PME101475     Target:  FOLR3     Tag:  C-6×His tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101474 efnb1 sp1

ECD Proteins

Human EFNB1 Protein, His Tag

SKU:  PME101474     Target:  EFNB1     Tag:  C-6×His tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101473 dll4 sp1

ECD Proteins

Human DLL4 Protein, His Tag

SKU:  PME101473     Target:  DLL4     Tag:  C-6×His tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101472 tpo sp1

ECD Proteins

Human TPO Protein, His Tag

SKU:  PME101472     Target:  TPO     Tag:  C-6×His tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101471 aplnr sp1

ECD Proteins

Human APLNR Protein, hFc Tag

SKU:  PME101471     Target:  APLNR     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101470 gpr84 sp1

ECD Proteins

Human GPR84 Protein, hFc Tag

SKU:  PME101470     Target:  GPR84     Tag:  C-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00