Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101465 cxcl14 sp1

ECD Proteins

Human CXCL14 Protein, hFc Tag

SKU:  PME101465     Target:  CXCL14     Tag:  C-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101464 ccl19 sp1

ECD Proteins

Human CCL19 Protein, hFc Tag

SKU:  PME101464     Target:  CCL19     Tag:  N-Human Fc tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101463 f9 sp1

ECD Proteins

Human F9 Protein, His Tag

SKU:  PME101463     Target:  F9     Tag:  C-6×His tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme101462 f10 sp1

ECD Proteins

Human F10 Protein, His Tag

SKU:  PME101462     Target:  F10     Tag:  C-6×His tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme101460 ntsr1 sp1

ECD Proteins

Human NTSR1 Protein, hFc Tag

SKU:  PME101460     Target:  NTSR1     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101459 clec10a sp1

ECD Proteins

Human CLEC10A Protein, hFc Tag

SKU:  PME101459     Target:  CLEC10A     Tag:  N-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme101458 uts2r sp1

ECD Proteins

Human UTS2R Protein, hFc Tag

SKU:  PME101458     Target:  UTS2R     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101457 fshb sp1

ECD Proteins

Human FSHB Protein, hFc Tag

SKU:  PME101457     Target:  FSHB     Tag:  C-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101456 ngal sp1

ECD Proteins

Human NGAL Protein, hFc Tag

SKU:  PME101456     Target:  NGAL     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101455 npy sp1

ECD Proteins

Human NPY Protein, hFc Tag

SKU:  PME101455     Target:  NPY     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101454 gip sp1

ECD Proteins

Human GIP Protein, hFc Tag

SKU:  PME101454     Target:  GIP     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101453 hmgb1 sp1

ECD Proteins

Human HMGB1 Protein, His Tag

SKU:  PME101453     Target:  HMGB1     Tag:  C-6×His tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme101452 c6 sp1

ECD Proteins

Human C6 Protein, His Tag

SKU:  PME101452     Target:  C6     Tag:  C-6×His tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101451 cadm3 sp1

ECD Proteins

Human CADM3 Protein, His Tag

SKU:  PME101451     Target:  CADM3     Tag:  C-6×His tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101450 ephb3 sp1

ECD Proteins

Human EPHB3 Protein, His Tag

SKU:  PME101450     Target:  EPHB3     Tag:  C-6×His tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme101449 pomc sp1

ECD Proteins

Human POMC Protein, His Tag

SKU:  PME101449     Target:  POMC     Tag:  C-6×His tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101448 mog sp1

ECD Proteins

Human MOG Protein, hFc Tag

SKU:  PME101448     Target:  MOG     Tag:  C-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme101446 ccl24 sp1

ECD Proteins

Human CCL24 Protein, hFc Tag

SKU:  PME101446     Target:  CCL24     Tag:  C-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme101445 cxcl10 sp1

ECD Proteins

Human CXCL10 Protein, hFc Tag

SKU:  PME101445     Target:  CXCL10     Tag:  N-Human Fc tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme101476 cd300c sp1

ECD Proteins

Human CD300C Protein, hFc Tag

SKU:  PME101476     Target:  CD300C     Tag:  C-Human Fc tag

Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00

sp-pme101461 ccl17 sp1

ECD Proteins

Human CCL17 Protein, hFc Tag

SKU:  PME101461     Target:  CCL17     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101444 ror1 sp1

ECD Proteins

Human ROR1(838-937) Protein, hFc Tag

SKU:  PME101444     Target:  ROR1     Tag:  N-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101443 her3 sp1

ECD Proteins

Human HER3(1243-1342) Protein, hFc Tag

SKU:  PME101443     Target:  HER3     Tag:  N-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101442 tim3 sp1

ECD Proteins

Human TIM3 Protein, hFc Tag

SKU:  PME101442     Target:  TIM3     Tag:  C-Human Fc tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme101441 mapt sp1

ECD Proteins

Human MAPT Protein, His Tag

SKU:  PME101441     Target:  MAPT     Tag:  N-6×His tag

Price: 10μg $96.00; 50μg $335.00 ; 100 μg $500.00

sp-pme101440 pedf sp1

ECD Proteins

Human PEDF Protein, His Tag

SKU:  PME101440     Target:  PEDF     Tag:  C-6×His tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101439 apoe sp1

ECD Proteins

Human APOE Protein, His Tag

SKU:  PME101439     Target:  APOE     Tag:  C-6×His tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101438 c9 sp1

ECD Proteins

Human C9 Protein, His Tag

SKU:  PME101438     Target:  C9     Tag:  C-6×His tag

Price: 10μg $96.00; 50μg $335.00 ; 100 μg $500.00

sp-pme101437 cd209 sp1

ECD Proteins

Human CD209 Protein, His Tag

SKU:  PME101437     Target:  CD209     Tag:  N-6×His tag

Price: 10μg $96.00; 50μg $335.00 ; 100 μg $500.00

sp-pme101436 cmklr1 sp1

ECD Proteins

Human CMKLR1 Protein, hFc Tag

SKU:  PME101436     Target:  CMKLR1     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101435 cd300a sp1

ECD Proteins

Human CD300A Protein, hFc Tag

SKU:  PME101435     Target:  CD300A     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101434 pth sp1

ECD Proteins

Human PTH Protein, hFc Tag

SKU:  PME101434     Target:  PTH     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00