Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101432 pld4 sp1

ECD Proteins

Human PLD4 Protein, hFc Tag

SKU:  PME101432     Target:  PLD4     Tag:  N-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101431 clec7a sp1

ECD Proteins

Human CLEC7A Protein, hFc Tag

SKU:  PME101431     Target:  CLEC7A     Tag:  N-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101430 ttr sp1

ECD Proteins

Human TTR Protein, hFc Tag

SKU:  PME101430     Target:  TTR     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101429 fstl3 sp1

ECD Proteins

Human FSTL3 Protein, hFc Tag

SKU:  PME101429     Target:  FSTL3     Tag:  C-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme101428 crth2 sp1

ECD Proteins

Human CRTH2 Protein, hFc Tag

SKU:  PME101428     Target:  CRTH2     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101427 cxcr6 sp1

ECD Proteins

Human CXCR6 Protein, hFc Tag

SKU:  PME101427     Target:  CXCR6     Tag:  C-Human Fc tag

Price: 10μg $90.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101426 bdkrb2 sp1

ECD Proteins

Human BDKRB2 Protein, hFc Tag

SKU:  PME101426     Target:  BDKRB2     Tag:  C-Human Fc tag

Price: 10μg $96.00; 50μg $335.00 ; 100 μg $500.00

sp-pme101404 cgrpr and ramp1 sp1

ECD Proteins

Human CGRPR and RAMP1 Heterodimer Protein, hFc Tag and mFc Tag

SKU:  PME101404     Target:  CGRPR and RAMP1     Tag:  C-Human Fc tag and C-mouse Fc tag

Price: 10μg $96.00; 50μg $335.00 ; 100 μg $500.00

sp-pme101383 cd33 sp1

ECD Proteins

Human CD33(18-259) Protein, His Tag

SKU:  PME101383     Target:  CD33     Tag:  C-10×His tag

Price: 10μg $95.00; 50μg $330.00 ; 100 μg $460.00

sp-pme101374 lingo1 sp1

ECD Proteins

Human LINGO1 Protein, His Tag

SKU:  PME101374     Target:  LINGO1     Tag:  C-6×His tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme100315 ki67 sp1

ECD Proteins

Human KI67(1206-1238) Protein, hFc Tag

SKU:  PME100315     Target:  KI67     Tag:  C-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme f100001 il31 sp1

ECD Proteins

Feline IL31 Protein, His Tag

SKU:  PME-F100001     Target:  IL31     Tag:  C-6×His tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme d100009 pd l1 sp1

ECD Proteins

Canine PD-L1 Protein, hFc Tag

SKU:  PME-D100009     Target:  PDL1     Tag:  C-Human Fc tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme d100008 pd 1 sp1

ECD Proteins

Canine PD-1 Protein, hFc Tag

SKU:  PME-D100008     Target:  PD-1     Tag:  C-Human Fc tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme d100007 pd l1 sp1

ECD Proteins

Canine PD-L1 Protein, mFc Tag

SKU:  PME-D100007      Target:  PDL1     Tag:  C-Mouse Fc tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme d100006 pd 1 sp1

ECD Proteins

Canine PD-1 Protein, mFc Tag

SKU:  PME-D100006     Target:  PD-1     Tag:  C-Mouse Fc tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme d100005 pd 1 sp1

ECD Proteins

Canine PD-1 Protein, His Tag

SKU:  PME-D100005     Target:  PD-1     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme c100056 lag3 sp1

ECD Proteins

Cynomolgus LAG3 Protein, hFc Tag

SKU:  PME-C100056     Target:  LAG3     Tag:  C-Human Fc tag

Price: 10μg $118.00; 50μg $409.00 ; 100 μg $615.00

sp-pme c100055 cd79b sp1

ECD Proteins

Cynomolgus CD79B Protein, hFc Tag

SKU:  PME-C100055     Target:  CD79B     Tag:  C-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme c100054 cd3d and cd3e sp1

ECD Proteins

Cynomolgus CD3D and CD3E Heterodimer Protein, His Tag and hFc Tag

SKU:  PME-C100054     Target:  CD3D and CD3E     Tag:  C-10×His tag and C-Human Fc tag

Price: 10μg $96.00; 50μg $335.00 ; 100 μg $500.00

sp-pme c100053 cd3d sp1

ECD Proteins

Cynomolgus CD3D Protein, His Tag

SKU:  PME-C100053     Target:  CD3D     Tag:  C-10×His tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme c100051 her3 sp1

ECD Proteins

Cynomolgus HER3 Protein, His Tag

SKU:  PME-C100051     Target:  HER3     Tag:  C-10×His tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme c100050 cldn18.2 sp1

ECD Proteins

Cynomolgus CLDN18.2 Protein, hFc Tag

SKU:  PME-C100050     Target:  CLDN18.2     Tag:  N-Human Fc tag

Price: 10μg $118.00; 50μg $409.00 ; 100 μg $615.00

sp-pme c100049 cxcr3 sp1

ECD Proteins

Cynomolgus CXCR3 Protein, hFc Tag

SKU:  PME-C100049     Target:  CXCR3     Tag:  C-Human Fc tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-pme c100048 cd47 sp1

ECD Proteins

Cynomolgus CD47 Protein, hFc Tag

SKU:  PME-C100048     Target:  CD47     Tag:  C-Human Fc tag

Price: 10μg $118.00; 50μg $409.00 ; 100 μg $615.00

sp-pme c100047 b7 h4 sp1

ECD Proteins

Cynomolgus B7-H4 Protein, His Tag

SKU:  PME-C100047     Target:  B7-H4     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $409.00 ; 100 μg $615.00

sp-pme c100046 trop2 sp1

ECD Proteins

Rhesus macaque Trop2 Protein, His Tag

SKU:  PME-C100046     Target:  Trop2     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $409.00 ; 100 μg $615.00

sp-pme c100045 nectin 4 sp1

ECD Proteins

Cynomolgus Nectin-4 Protein, His Tag

SKU:  PME-C100045     Target:  Nectin-4     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme c100044 cdh6 sp1

ECD Proteins

Cynomolgus CDH6 Protein, His Tag

SKU:  PME-C100044     Target:  CDH6     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme c100043 cd73 sp1

ECD Proteins

Cynomolgus CD73 Protein, His Tag

SKU:  PME-C100043     Target:  CD73     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme c100042 fap sp1

ECD Proteins

Cynomolgus FAP Protein, His Tag

SKU:  PME-C100042     Target:  FAP     Tag:  N-10×His tag

Price: 10μg $118.00; 50μg $409.00 ; 100 μg $615.00

sp-pme c100041 tim3 sp1

ECD Proteins

Cynomolgus TIM3 Protein, hFc Tag

SKU:  PME-C100041     Target:  TIM3     Tag:  C-Human Fc tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00