Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme c100040 folr1 sp1

ECD Proteins

Cynomolgus FOLR1 Protein, His Tag

SKU:  PME-C100040     Target:  FOLR1     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $409.00 ; 100 μg $615.00

sp-pme c100039 mesothelin sp1

ECD Proteins

Cynomolgus Mesothelin Protein, His Tag

SKU:  PME-C100039     Target:  Mesothelin     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $409.00 ; 100 μg $615.00

sp-pme c100034 micb sp1

ECD Proteins

Cynomolgus MICB Protein, His Tag

SKU:  PME-C100034     Target:  MICB     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme c100027 mica sp1

ECD Proteins

Cynomolgus MICA Protein, His Tag

SKU:  PME-C100027     Target:  MICA     Tag:  C-10×His tag

Price: 10μg $118.00; 50μg $429.00 ; 100 μg $645.00

sp-pme d100004 il31 sp1

ECD Proteins

Canine IL31 Protein, His Tag

SKU:  PME-D100004     Target:  IL31     Tag:  C-6×His tag

Price: 10μg $100.00; 50μg $358.00 ; 100 μg $528.00

sp-PME C100052 CD30 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CD30 Protein, His Tag

SKU:  PME-C100052     Target:  CD30     Tag:  C-10×His tag

Price:10μg $129.00; 50μg $476.00; 100μg $714.00

sp-PME C100038 TIGIT Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus TIGIT Protein, hFc Tag

SKU:  PME-C100038     Target:  TIGIT     Tag:  C-Human Fc tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100037 CD3E Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CD3E Protein, hFc Tag

SKU:  PME-C100037     Target:  CD3E     Tag:  C-Human Fc tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100036 EGFR Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus EGFR Protein, His Tag

SKU:  PME-C100036     Target:  EGFR     Tag:  C-10×His tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100035 PSMA Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus PSMA Protein, His Tag

SKU:  PME-C100035     Target:  PSMA     Tag:  N-10×His tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100033 CEACAM5 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CEACAM5 Protein, His Tag

SKU:  PME-C100033     Target:  CEACAM5     Tag:  C-10×His tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100032 CA9 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CA9 Protein, His Tag

SKU:  PME-C100032     Target:  CA9     Tag:  C-10×His tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00

sp-PME C100031 B7 H6 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus B7-H6 Protein, His Tag

SKU:  PME-C100031     Target:  B7-H6     Tag:  C-10×His tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00

sp-PME C100030 CD7 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CD7 Protein, hFc Tag

SKU:  PME-C100030     Target:  CD7     Tag:  C-Human Fc tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100029 CDH17 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CDH17 Protein, His Tag

SKU:  PME-C100029     Target:  CDH17     Tag:  C-10×His tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00

sp-PME C100028 CD24 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CD24 Protein, hFc Tag

SKU:  PME-C100028     Target:  CD24     Tag:  C-Human Fc tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100025 AXL Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus AXL Protein, His Tag

SKU:  PME-C100025     Target:  AXL     Tag:  C-10×His tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100024 TREM2 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus TREM2 Protein, hFc Tag

SKU:  PME-C100024     Target:  TREM2     Tag:  C-Human Fc tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100023 4 1BB Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus 4-1BB Protein, hFc Tag

SKU:  PME-C100023     Target:  4-1BB     Tag:  C-Human Fc tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100022 GUCY2C Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus GUCY2C Protein, His Tag

SKU:  PME-C100022     Target:  GUCY2C     Tag:  C-10×His tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00

sp-PME C100021 PD L1 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus PD-L1 Protein, His Tag

SKU:  PME-C100021     Target:  PDL1     Tag:  C-10×His tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100020 5T4 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus 5T4 Protein, His Tag

SKU:  PME-C100020     Target:  5T4     Tag:  C-10×His tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100019 TNFSF15 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus TNFSF15 Protein, hFc Tag

SKU:  PME-C100019     Target:  TNFSF15     Tag:  N-Human Fc tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00

sp-PME C100018 B7 H3 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus B7-H3 Protein, His Tag

SKU:  PME-C100018     Target:  B7-H3     Tag:  C-10×His tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-PME C100017 ALPP Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus ALPP Protein, His Tag

SKU:  PME-C100017     Target:  ALPP     Tag:  C-10×His tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00

sp-PME M100111 PROM1 Fig.1 SDS PAGE 1

ECD Proteins

Mouse PROM1 Protein, hFc Tag

SKU:  PME-M100111     Target:  PROM1     Tag:  C-Human Fc tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100110 IL23A Fig.1 SDS PAGE 1

ECD Proteins

Mouse IL23A Protein, hFc Tag

SKU:  PME-M100110     Target:  IL23A     Tag:  C-Human Fc tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME M100109 GIPR Fig.1 SDS PAGE 1

ECD Proteins

Mouse GIPR Protein, hFc Tag

SKU:  PME-M100109     Target:  GIPR     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100108 NECTIN 4 Fig.1 SDS PAGE 1

ECD Proteins

Mouse Nectin-4 Protein, His Tag

SKU:  PME-M100108     Target:  Nectin-4     Tag:  C-10×His tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100107 CCR2 Fig.1 SDS PAGE 1

ECD Proteins

Mouse CCR2 Protein, hFc Tag

SKU:  PME-M100107     Target:  CCR2     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100106 CCR6 Fig.1 SDS PAGE 1

ECD Proteins

Mouse CCR6 Protein, hFc Tag

SKU:  PME-M100106     Target:  CCR6     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100105 CD98 Fig.1 SDS PAGE 1

ECD Proteins

Mouse CD98 Protein, His Tag

SKU:  PME-M100105     Target:  CD98     Tag:  N-10×His tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00