Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-PME M100104 TFRC Fig.1 SDS PAGE 1

ECD Proteins

Mouse TFRC Protein, His Tag

SKU:  PME-M100104     Target:  TFRC     Tag:  N-10×His tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME M100103 PSMA Fig.1 SDS PAGE 1

ECD Proteins

Mouse PSMA Protein, His Tag

SKU:  PME-M100103     Target:  PSMA     Tag:  N-10×His tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101495 FCRL5 Fig.1 SDS PAGE 1

ECD Proteins

Human FCRL5(745-847)Protein, mFc Tag

SKU:  PME101495     Target:  FCRL5     Tag:  C-mouse Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101494 FCRL5 Fig.1 SDS PAGE 1

ECD Proteins

Human FCRL5(745-850)Protein, hFc Tag

SKU:  PME101494     Target:  FCRL5     Tag:  N-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101493 FCRL5 Fig.1 SDS PAGE 1

ECD Proteins

Human FCRL5(745-847)Protein, hFc Tag

SKU:  PME101493     Target:  FCRL5     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101424 XCR1 Fig.1 SDS PAGE 1

ECD Proteins

Human XCR1 Protein, hFc Tag

SKU:  PME101424     Target:  XCR1     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101423 BAMBI Fig.1 SDS PAGE 1

ECD Proteins

Human BAMBI Protein, hFc Tag

SKU:  PME101423     Target:  BAMBI     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101422 CCL3 Fig.1 SDS PAGE 1

ECD Proteins

Human CCL3 Protein, hFc Tag

SKU:  PME101422     Target:  CCL3     Tag:  C-Human Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-PME101421 CCL5 Fig.1 SDS PAGE 1

ECD Proteins

Human CCL5 Protein, hFc Tag

SKU:  PME101421     Target:  CCL5     Tag:  C-Human Fc tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101420 IFNA2 Fig.1 SDS PAGE 1

ECD Proteins

Human IFNA2 Protein, hFc Tag

SKU:  PME101420     Target:  IFNA2     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101419 CRCP Fig.1 SDS PAGE 1

ECD Proteins

Human CRCP Protein, hFc Tag

SKU:  PME101419     Target:  CRCP     Tag:  N-Human Fc tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101418 ADAM17 Fig.1 SDS PAGE 1

ECD Proteins

Human ADAM17(581-642) Protein, mFc Tag

SKU:  PME101418     Target:  ADAM17     Tag:  C-mouse Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101417 LEP Fig.1 SDS PAGE 1

ECD Proteins

Human LEP Protein, hFc Tag

SKU:  PME101417     Target:  LEP     Tag:  C-Human Fc tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101416 ADIPOQ Fig.1 SDS PAGE 1

ECD Proteins

Human ADIPOQ Protein, hFc Tag

SKU:  PME101416     Target:  ADIPOQ     Tag:  C-Human Fc tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101415 APOA2 Fig.1 SDS PAGE 1

ECD Proteins

Human APOA2 Protein, hFc Tag

SKU:  PME101415     Target:  APOA2     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101414 GAL Fig.1 SDS PAGE 1

ECD Proteins

Human GAL Protein, hFc Tag

SKU:  PME101414     Target:  GAL     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101413 ADGRD1 Fig.1 SDS PAGE 1

ECD Proteins

Human ADGRD1 Protein, His Tag

SKU:  PME101413     Target:  ADGRD1     Tag:  C-6×His tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101412 HER3 Fig.1 SDS PAGE 1

ECD Proteins

Human HER3(544-643) Protein, mFc Tag

SKU:  PME101412     Target:  HER3     Tag:  C-mouse Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101411 HER3 Fig.1 SDS PAGE 1

ECD Proteins

Human HER3(544-643) Protein, hFc Tag

SKU:  PME101411     Target:  HER3     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101410 OR2H1 Fig.1 SDS PAGE 1

ECD Proteins

Human OR2H1 Protein, hFc Tag

SKU:  PME101410     Target:  OR2H1     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101408 FGFR2IIIb Fig.1 SDS PAGE 1

ECD Proteins

Human FGFR2b Protein, His Tag

SKU:  PME101408     Target:  FGFR2     Tag:  C-10×His tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101407 KLK1 Fig.1 SDS PAGE 1

ECD Proteins

Human KLK1 Protein, His Tag

SKU:  PME101407     Target:  KLK1     Tag:  C-6×His tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101406 IL31RA Fig.1 SDS PAGE 1

ECD Proteins

Human IL31RA Protein, His Tag

SKU:  PME101406     Target:  IL31RA     Tag:  C-6×His tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101405 MET Fig.1 SDS PAGE 1

ECD Proteins

Human MET Protein, His Tag

SKU:  PME101405     Target:  MET     Tag:  C-10×His tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101403 GLP2R Fig.1 SDS PAGE 1

ECD Proteins

Human GLP2R Protein, His Tag

SKU:  PME101403     Target:  GLP2R     Tag:  C-6×His tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101402 TIM4 Fig.1 SDS PAGE 1

ECD Proteins

Human TIM4 Protein, His Tag

SKU:  PME101402     Target:  TIM4     Tag:  C-6×His tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101401 ADGRE5 Fig.1 SDS PAGE 1

ECD Proteins

Human ADGRE5 Protein, His Tag

SKU:  PME101401     Target:  ADGRE5     Tag:  C-6×His tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101396 CFI Fig.1 SDS PAGE 1

ECD Proteins

Human CFI Protein, His Tag

SKU:  PME101396     Target:  CFI     Tag:  C-6×His tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101395 PSAP Fig.1 SDS PAGE 1

ECD Proteins

Human SAP Protein, His Tag

SKU:  PME101395     Target:  SAP     Tag:  C-6×His tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101394 C1S Fig.1 SDS PAGE 1

ECD Proteins

Human C1S Protein, His Tag

SKU:  PME101394     Target:  C1S     Tag:  C-6×His tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101393 DR6 Fig.1 SDS PAGE 1

ECD Proteins

Human DR6 Protein, His Tag

SKU:  PME101393     Target:  DR6     Tag:  C-6×His tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101392 FCN1 Fig.1 SDS PAGE 1

ECD Proteins

Human FCN1 Protein, His Tag

SKU:  PME101392     Target:  FCN1     Tag:  C-6×His tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00