Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-PME101409 MET Fig.1 SDS PAGE 1

ECD Proteins

Human MET Protein, hFc Tag

SKU:  PME101409     Target:  MET     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101357 MICAa3 Fig.1 SDS PAGE 1

ECD Proteins

Human MICAa3 Protein, His Tag

SKU:  PME101357     Target:  MICAa3     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101356 MICAa3 Fig.1 SDS PAGE 1

ECD Proteins

Human MICAa3 Protein, mFc Tag

SKU:  PME101356     Target:  MICAa3     Tag:  C-mouse Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101355 APLN Fig.1 SDS PAGE 1

ECD Proteins

Human APLN Protein, hFc Tag

SKU:  PME101355     Target:  APLN     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101354 F2RL3 Fig.1 SDS PAGE 1

ECD Proteins

Human F2RL3 Protein, hFc Tag

SKU:  PME101354     Target:  F2RL3     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101353 GPR132 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR132 Protein, hFc Tag

SKU:  PME101353     Target:  GPR132     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101352 IL25 Fig.1 SDS PAGE 1

ECD Proteins

Human IL25 Protein, hFc Tag

SKU:  PME101352     Target:  IL25     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101351 CD62E Fig.1 SDS PAGE 1

ECD Proteins

Human CD62E Protein, His Tag

SKU:  PME101351     Target:  CD62E     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101349 TREML1 Fig.1 SDS PAGE 1

ECD Proteins

Human TREML1 Protein, hFc Tag

SKU:  PME101349     Target:  TREML1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101348 CFH Fig.1 SDS PAGE 1

ECD Proteins

Human CFH Protein, His Tag

SKU:  PME101348     Target:  CFH     Tag:  C-10×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101347 C5 Fig.1 SDS PAGE 1

ECD Proteins

Human C5 Protein, His Tag

SKU:  PME101347     Target:  C5     Tag:  C-10×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101346 TREML2 Fig.1 SDS PAGE 1

ECD Proteins

Human TREML2 Protein, His Tag

SKU:  PME101346     Target:  TREML2     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101345 LEPR Fig.1 SDS PAGE 1

ECD Proteins

Human LEPR Protein, His Tag

SKU:  PME101345     Target:  LEPR     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101344 APOH Fig.1 SDS PAGE 1

ECD Proteins

Human APOH Protein, His Tag

SKU:  PME101344     Target:  APOH     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101343 CX3CL1 Fig.1 SDS PAGE 1

ECD Proteins

Human CX3CL1 Protein, His Tag

SKU:  PME101343     Target:  CX3CL1     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101342 C2 Fig.1 SDS PAGE 1

ECD Proteins

Human C2 Protein, His Tag

SKU:  PME101342     Target:  C2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101341 MAG Fig.1 SDS PAGE 1

ECD Proteins

Human MAG Protein, His Tag

SKU:  PME101341     Target:  MAG     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101340 MFAP4 Fig.1 SDS PAGE 1

ECD Proteins

Human MFAP4 Protein, His Tag

SKU:  PME101340     Target:  MFAP4     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101339 AOC3 Fig.1 SDS PAGE 1

ECD Proteins

Human AOC3 Protein, His Tag

SKU:  PME101339     Target:  AOC3     Tag:  N-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101338 MSR1 Fig.1 SDS PAGE 1

ECD Proteins

Human MSR1 Protein, His Tag

SKU:  PME101338     Target:  MSR1     Tag:  N-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101337 FGFR2 Fig.1 SDS PAGE 1

ECD Proteins

Human FGFR2c Protein, His Tag

SKU:  PME101337     Target:  FGFR2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101336 KIR3DL3 Fig.1 SDS PAGE 1

ECD Proteins

Human KIR3DL3 Protein, His Tag

SKU:  PME101336     Target:  KIR3DL3     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101335 NTRK2 Fig.1 SDS PAGE 1

ECD Proteins

Human NTRK2 Protein, His Tag

SKU:  PME101335     Target:  NTRK2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101334 FGFR1 Fig.1 SDS PAGE 1

ECD Proteins

Human FGFR1 Protein, His Tag

SKU:  PME101334     Target:  FGFR1     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101333 RTN4R Fig.1 SDS PAGE 1

ECD Proteins

Human RTN4R Protein, His Tag

SKU:  PME101333     Target:  RTN4R     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101332 CCL24 Fig.1 SDS PAGE 1

ECD Proteins

Human CCL24 Protein, hFc Tag

SKU:  PME101332     Target:  CCL24     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101331 PIP Fig.1 SDS PAGE 1

ECD Proteins

Human PIP Protein, hFc Tag

SKU:  PME101331     Target:  PIP     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME100344 MERTK Fig.1 SDS PAGE 1

ECD Proteins

Human MERTK Protein, His Tag

SKU:  PME100344     Target:  MERTK     Tag:  C-10×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME100199 CDH17 Fig.1 SDS PAGE 1

ECD Proteins

Human CDH17(567-667) Protein, hFc Tag

SKU:  PME100199     Target:  CDH17     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101350 CD161 Fig.1 SDS PAGE 1

ECD Proteins

Human CD161 Protein, hFc Tag

SKU:  PME101350     Target:  CD161     Tag:  N-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME M100102 TNFSF15 Fig.1 SDS PAGE 1

ECD Proteins

Mouse TNFSF15 Protein, hFc Tag

SKU:  PME-M100102     Target:  TNFSF15     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100101 IGF 1R Fig.1 SDS PAGE 1

ECD Proteins

Mouse IGF-1R Protein, His Tag

SKU:  PME-M100101     Target:  IGF-1R     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00