Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-PME M100100 IL6 Fig.1 SDS PAGE 1

ECD Proteins

Mouse IL6 Protein, hFc Tag

SKU:  PME-M100100     Target:  IL6     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100099 PD 1 Fig.1 SDS PAGE 1

ECD Proteins

Mouse PD-1 Protein, hFc Tag

SKU:  PME-M100099     Target:  PD-1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100098 CDH17 Fig.1 SDS PAGE 1

ECD Proteins

Mouse CDH17 Protein, His Tag

SKU:  PME-M100098     Target:  CDH17     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100097 ROR2 Fig.1 SDS PAGE 1

ECD Proteins

Mouse ROR2 Protein, His Tag

SKU:  PME-M100097     Target:  ROR2     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100096 IGF1 Fig.1 SDS PAGE 1

ECD Proteins

Mouse IGF1 Protein, hFc Tag

SKU:  PME-M100096     Target:  IGF1     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100095 CD70 Fig.1 SDS PAGE 1

ECD Proteins

Mouse CD70 Protein, hFc Tag

SKU:  PME-M100095     Target:  CD70     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100094 AXL Fig.1 SDS PAGE 1

ECD Proteins

Mouse AXL Protein, His Tag

SKU:  PME-M100094     Target:  AXL     Tag:  C-10×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100093 CGRP Fig.1 SDS PAGE 1

ECD Proteins

Mouse CGRP Protein, hFc Tag

SKU:  PME-M100093     Target:  CGRP     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100092 AFP Fig.1 SDS PAGE 1

ECD Proteins

Mouse AFP Protein, His Tag

SKU:  PME-M100092     Target:  AFP     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100091 HistoneH1.2 Fig.1 SDS PAGE 1

ECD Proteins

Mouse HistoneH1.2 Protein, hFc Tag

SKU:  PME-M100091     Target:  HistoneH1.2     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100090 TSLP Fig.1 SDS PAGE 1

ECD Proteins

Mouse TSLP Protein, hFc Tag

SKU:  PME-M100090     Target:  TSLP     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100089 5T4 Fig.1 SDS PAGE 1

ECD Proteins

Mouse 5T4 Protein, His Tag

SKU:  PME-M100089     Target:  5T4     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100088 IL11RA Fig.1 SDS PAGE 1

ECD Proteins

Mouse IL11RA Protein, His Tag

SKU:  PME-M100088     Target:  IL11RA     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100087 GUCY2C Fig.1 SDS PAGE 1

ECD Proteins

Mouse GUCY2C Protein, His Tag

SKU:  PME-M100087     Target:  GUCY2C     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100086 TNFRSF1B Fig.1 SDS PAGE 1

ECD Proteins

Mouse TNFRSF1B Protein, His Tag

SKU:  PME-M100086     Target:  TNFRSF1B     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100085 CXADR Fig.1 SDS PAGE 1

ECD Proteins

Mouse CXADR Protein, His Tag

SKU:  PME-M100085     Target:  CXADR     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100084 EPHA2 Fig.1 SDS PAGE 1

ECD Proteins

Mouse EPHA2 Protein, His Tag

SKU:  PME-M100084     Target:  EPHA2     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100083 SEZ6 Fig.1 SDS PAGE 1

ECD Proteins

Mouse SEZ6 Protein, His Tag

SKU:  PME-M100083     Target:  SEZ6     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100082 ICAM1 Fig.1 SDS PAGE 1

ECD Proteins

Mouse ICAM1 Protein, His Tag

SKU:  PME-M100082     Target:  ICAM1     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100081 CD45 Fig.1 SDS PAGE 1

ECD Proteins

Mouse CD45 Protein, His Tag

SKU:  PME-M100081     Target:  CD45     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME M100080 TREM2 Fig.1 SDS PAGE 1

ECD Proteins

Mouse TREM2 Protein, hFc Tag

SKU:  PME-M100080     Target:  TREM2     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100079 CD24 Fig.1 SDS PAGE 1

ECD Proteins

Mouse CD24 Protein, hFc Tag

SKU:  PME-M100079     Target:  CD24     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME M100078 B7 H4 Fig.1 SDS PAGE 1

ECD Proteins

Mouse B7-H4 Protein, His Tag

SKU:  PME-M100078     Target:  B7-H4     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME M100077 PD L1 Fig.1 SDS PAGE 1

ECD Proteins

Mouse PD-L1 Protein, hFc Tag

SKU:  PME-M100077     Target:  PDL1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101400 PLA2R1 Fig.1 SDS PAGE 1

ECD Proteins

Human PLA2R1(21-164) Protein, hFc Tag

SKU:  PME101400     Target:  PLA2R1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101399 PLA2R1 Fig.1 SDS PAGE 1

ECD Proteins

Human PLA2R1(21-521+1097-1246) Protein, His Tag

SKU:  PME101399     Target:  PLA2R1     Tag:  C-10×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101398 PLA2R1 Fig.1 SDS PAGE 1

ECD Proteins

Human PLA2R1(21-164+223-359+1097-1246) Protein, His Tag

SKU:  PME101398     Target:  PLA2R1     Tag:  C-10×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101397 PLA2R1 Fig.1 SDS PAGE 1 1

ECD Proteins

Human PLA2R1 Protein, His Tag

SKU:  PME101397     Target:  PLA2R1     Tag:  C-10×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101330 IGF1 Fig.1 SDS PAGE 1

ECD Proteins

Human IGF1 Protein, hFc Tag

SKU:  PME101330     Target:  IGF1     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101381 HBSAG Fig.1 SDS PAGE 1

ECD Proteins

Hepatitis B virus HBSAG Protein, hFc Tag

SKU:  PME101381     Target:  HBSAG     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101329 WFDC1 Fig.1 SDS PAGE 1

ECD Proteins

Human WFDC1 Protein, hFc Tag

SKU:  PME101329     Target:  WFDC1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101328 MXRA8 Fig.1 SDS PAGE 1

ECD Proteins

Human MXRA8 Protein, His Tag

SKU:  PME101328     Target:  MXRA8     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00