Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-PME101327 LILRB5 Fig.1 SDS PAGE 1

ECD Proteins

Human LILRB5 Protein, His Tag

SKU:  PME101327     Target:  LILRB5     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101326 LILRA2 Fig.1 SDS PAGE 1

ECD Proteins

Human LILRA2 Protein, His Tag

SKU:  PME101326     Target:  LILRA2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101325 IL13RA2 Fig.1 SDS PAGE 1

ECD Proteins

Human IL13RA2 Protein, hFc Tag

SKU:  PME101325     Target:  IL13RA2     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101324 VWF Fig.1 SDS PAGE 1

ECD Proteins

Human VWF(1596-1668) Protein, hFc Tag

SKU:  PME101324     Target:  VWF     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101323 GPR65 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR65 Protein, hFc Tag

SKU:  PME101323     Target:  GPR65     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101322 CD79A Fig.1 SDS PAGE 1

ECD Proteins

Human CD79A Protein, hFc Tag

SKU:  PME101322     Target:  CD79A     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101321 EGFRVIII Fig.1 SDS PAGE 1

ECD Proteins

Human EGFRVIII Protein, His Tag

SKU:  PME101321     Target:  EGFRVIII     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101320 ENPP2 Fig.1 SDS PAGE 1

ECD Proteins

Human ENPP2 Protein, His Tag

SKU:  PME101320     Target:  ENPP2     Tag:  N-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101319 CSPG5 Fig.1 SDS PAGE 1

ECD Proteins

Human CSPG5 Protein, His Tag

SKU:  PME101319     Target:  CSPG5     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101318 IL1RL2 Fig.1 SDS PAGE 1

ECD Proteins

Human IL1RL2 Protein, His Tag

SKU:  PME101318     Target:  IL1RL2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101317 SEMA7A Fig.1 SDS PAGE 1

ECD Proteins

Human SEMA7A Protein, His Tag

SKU:  PME101317     Target:  SEMA7A     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101316 PLAT Fig.1 SDS PAGE 1

ECD Proteins

Human PLAT Protein, His Tag

SKU:  PME101316     Target:  PLAT     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101315 CCL11 Fig.1 SDS PAGE 1

ECD Proteins

Human CCL11 Protein, hFc Tag

SKU:  PME101315     Target:  CCL11     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101314 CFD Fig.1 SDS PAGE 1

ECD Proteins

Human CFD Protein, hFc Tag

SKU:  PME101314     Target:  CFD     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101313 CLEC5A Fig.1 SDS PAGE 1

ECD Proteins

Human CLEC5A Protein, hFc Tag

SKU:  PME101313     Target:  CLEC5A     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101312 LOX 1 Fig.1 SDS PAGE 1

ECD Proteins

Human LOX-1 Protein, hFc Tag

SKU:  PME101312     Target:  LOX-1     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101311 LILRB4 Fig.1 SDS PAGE 1

ECD Proteins

Human LILRB4 Protein, hFc Tag

SKU:  PME101311     Target:  LILRB4     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101310 CLEC4C Fig.1 SDS PAGE 1

ECD Proteins

Human CLEC4C Protein, His Tag

SKU:  PME101310     Target:  CLEC4C     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101308 IGSF11 Fig.1 SDS PAGE 1

ECD Proteins

Human IGSF11 Protein, hFc Tag

SKU:  PME101308     Target:  IGSF11     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101307 IL4 Fig.1 SDS PAGE 1

ECD Proteins

Human IL4 Protein, mFc Tag

SKU:  PME101307     Target:  IL4     Tag:  C-mouse Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101306 CD98 Fig.1 SDS PAGE 1

ECD Proteins

Human CD98 Protein, His Tag

SKU:  PME101306     Target:  CD98     Tag:  N-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101304 PAI1 Fig.1 SDS PAGE 1

ECD Proteins

Human PAI1 Protein, His Tag

SKU:  PME101304     Target:  PAI1     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101303 MELTF Fig.1 SDS PAGE 1

ECD Proteins

Human MELTF Protein, His Tag

SKU:  PME101303     Target:  MELTF     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101292 GPR64 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR64 Protein, His Tag

SKU:  PME101292     Target:  GPR64     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME100319 KIR2DL3 Fig.1 SDS PAGE 1

ECD Proteins

Human KIR2DL3 Protein, His Tag

SKU:  PME100319     Target:  KIR2DL3     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME100213 CRLF2 Fig.1 SDS PAGE 1

ECD Proteins

Human CRLF2 Protein, His Tag

SKU:  PME100213     Target:  CRLF2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

elisa-PME30021 C687 P08476 ELISA 0

ECD Proteins

Human/Mouse/Rat Activin A Protein

SKU:  PME30021     Target:  Activin     Tag: 

Price: 50 μg $503.00

sp-PME C100016 SELPLG Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus SELPLG Protein, His Tag

SKU:  PME-C100016     Target:  SELPLG     Tag:  C-6×His Tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00

sp-PME C100015 IL18BP Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus IL18BP Protein, His Tag

SKU:  PME-C100015     Target:  IL18BP     Tag:  C-6×His Tag

Price: 10μg $100.00 ; 50μg $375.00 ; 100 μg $550.00

sp-PME C100014 CD93 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CD93 Protein, His Tag

SKU:  PME-C100014     Target:  CD93     Tag:  C-6×His Tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00

sp-PME C100013 CLEC4C Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CLEC4C Protein, His Tag

SKU:  PME-C100013     Target:  CLEC4C     Tag:  N-6×His Tag

Price: 10μg $100.00 ; 50μg $375.00 ; 100 μg $550.00

sp-PME C100012 ADAM9 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus ADAM9 Protein, His Tag

SKU:  PME-C100012     Target:  ADAM9     Tag:  C-6×His Tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00