Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-PME101271 HAMP Fig.1 SDS PAGE 1

ECD Proteins

Human HAMP(65-84) Protein, hFc Tag

SKU:  PME101271     Target:  HAMP     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101270 TMEM219 Fig.1 SDS PAGE 1

ECD Proteins

Human TMEM219 Protein, hFc Tag

SKU:  PME101270     Target:  TMEM219     Tag:  N-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101268 GRPR Fig.1 SDS PAGE 1

ECD Proteins

Human GRPR Protein, hFc Tag

SKU:  PME101268     Target:  GRPR     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101267 GIPR Fig.1 SDS PAGE 1

ECD Proteins

Human GIPR Protein, hFc Tag

SKU:  PME101267     Target:  GIPR     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101266 IL12AIL12B Fig.1 SDS PAGE 1

ECD Proteins

Human IL12A and IL12B Heterodimer Protein, hFc Tag and His Tag

SKU:  PME101266     Target:  IL12A and IL12B     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101265 TNFRSF1B Fig.1 SDS PAGE 1

ECD Proteins

Human TNFRSF1B Protein, His Tag

SKU:  PME101265     Target:  TNFRSF1B     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101263 LRRN1 Fig.1 SDS PAGE 1

ECD Proteins

Human LRRN1 Protein, His Tag

SKU:  PME101263     Target:  LRRN1     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101262 CLEC4A Fig.1 SDS PAGE 1

ECD Proteins

Human CLEC4A Protein, hFc Tag

SKU:  PME101262     Target:  CLEC4A     Tag:  N-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101261 MRGPRX2 Fig.1 SDS PAGE 1

ECD Proteins

Human MRGPRX2 Protein, hFc Tag

SKU:  PME101261     Target:  MRGPRX2     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101260 IGHE Fig.1 SDS PAGE 1

ECD Proteins

Human IGHE Protein, His Tag

SKU:  PME101260     Target:  IgHE     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101258 VSIG1 Fig.1 SDS PAGE 1

ECD Proteins

Human VSIG1 Protein, His Tag

SKU:  PME101258     Target:  VSIG1     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101257 ICAM3 Fig.1 SDS PAGE 1

ECD Proteins

Human ICAM3 Protein, His Tag

SKU:  PME101257     Target:  ICAM3     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101256 FCRL2 Fig.1 SDS PAGE 1

ECD Proteins

Human FCRL2 Protein, His Tag

SKU:  PME101256     Target:  FCRL2     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101036 CALCA Fig.1 SDS PAGE 1

ECD Proteins

Human CALCA(85-141) Protein, hFc Tag

SKU:  PME101036     Target:  CALCA     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME100317 KIR2DL1 Fig.1 SDS PAGE 1

ECD Proteins

Human KIR2DL1 Protein, hFc Tag

SKU:  PME100843     Target:  KIR2DL1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101269 GLP1R Fig.1 SDS PAGE 1

ECD Proteins

Human GLP1R Protein, hFc Tag

SKU:  PME101269     Target:  GLP1R     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101264 LRP10 Fig.1 SDS PAGE 1

ECD Proteins

Human LRP10 Protein, His Tag

SKU:  PME101264     Target:  LRP10     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101254 IL23R Fig.1 SDS PAGE 1

ECD Proteins

Human IL23R Protein, His Tag

SKU:  PME101254     Target:  IL23R     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101253 KREMEN2 Fig.1 SDS PAGE 1

ECD Proteins

Human KREMEN2 Protein, His Tag

SKU:  PME101253     Target:  KREMEN2     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101251 LY6H Fig.1 SDS PAGE 1

ECD Proteins

Human LY6H Protein, hFc Tag

SKU:  PME101251     Target:  LY6H     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101250 IL19 Fig.1 SDS PAGE 1

ECD Proteins

Human IL19 Protein, hFc Tag

SKU:  PME101250     Target:  IL19     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101249 ninj1 sp1

ECD Proteins

Human NINJ1 Protein, hFc Tag

SKU:  PME101249     Target:  NINJ1     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101248 IL20RA Fig.1 SDS PAGE 1

ECD Proteins

Human IL20RA Protein, hFc Tag

SKU:  PME101248     Target:  IL20RA     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101245 DKK1 Fig.1 SDS PAGE 1

ECD Proteins

Human DKK1 Protein, mFc Tag

SKU:  PME101245     Target:  DKK1     Tag:  C-mouse Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101244 RHOC Fig.1 SDS PAGE 1

ECD Proteins

Human RHOC(1-189) Protein, hFc Tag

SKU:  PME101244     Target:  RhoC     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101243 RHOB Fig.1 SDS PAGE 1

ECD Proteins

Human RHOB Protein, hFc Tag

SKU:  PME101243     Target:  RHOB     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101241 CXADR Fig.1 SDS PAGE 1

ECD Proteins

Human CXADR Protein, His Tag

SKU:  PME101241     Target:  CXADR     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101240 APLP2 Fig.1 SDS PAGE 1

ECD Proteins

Human APLP2 Protein, His Tag

SKU:  PME101240     Target:  APLP2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101239 DKK1 Fig.1 SDS PAGE 1

ECD Proteins

Human DKK1 Protein, His Tag

SKU:  PME101239     Target:  DKK1     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101237 TGFA Fig.1 SDS PAGE 1

ECD Proteins

Human TGFA Protein, hFc Tag

SKU:  PME101057     Target:  TGFA     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $256.00 ; 100μg $385.00

sp-PME101236 CCL19 Fig.1 SDS PAGE 1

ECD Proteins

Human CCL19 Protein, hFc Tag

SKU:  PME101236     Target:  CCL19     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101235 BTC Fig.1 SDS PAGE 1

ECD Proteins

Human BTC Protein, hFc Tag

SKU:  PME101235     Target:  BTC     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00