Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-PME101302 GPR81 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR81 Protein, hFc Tag

SKU:  PME101302     Target:  GPR81     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101301 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(110-139) Protein, hFc Tag

SKU:  PME101301     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101300 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(77-106) Protein, hFc Tag

SKU:  PME101300     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101299 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(44-72) Protein, hFc Tag

SKU:  PME101299     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101298 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(11-40) Protein, hFc Tag

SKU:  PME101298     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101297 IBSP Fig.1 SDS PAGE 1

ECD Proteins

Human IBSP Protein, His Tag

SKU:  PME101297     Target:  IBSP     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101296 IL4 Fig.1 SDS PAGE 1

ECD Proteins

Human IL4 Protein, His Tag

SKU:  PME101296     Target:  IL4     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101295 ARTN Fig.1 SDS PAGE 1

ECD Proteins

Human ARTN Protein, hFc Tag

SKU:  PME101295     Target:  ARTN     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101294 S100A9 Fig.1 SDS PAGE 1

ECD Proteins

Human S100A9 Protein, hFc Tag

SKU:  PME101294     Target:  S100A9     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101291 FSTL1 Fig.1 SDS PAGE 1

ECD Proteins

Human FSTL1 Protein, His Tag

SKU:  PME101291     Target:  FSTL1     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101289 CALCA Fig.1 SDS PAGE 1

ECD Proteins

Human CALCA(26-141) Protein, hFc Tag

SKU:  PME101289     Target:  CALCA     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101288 CXCL5 Fig.1 SDS PAGE 1

ECD Proteins

Human CXCL5 Protein, hFc Tag

SKU:  PME101288     Target:  CXCL5     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101287 DSG2 Fig.1 SDS PAGE 1

ECD Proteins

Human DSG2(24-609) Protein, His Tag

SKU:  PME101287     Target:  DSG2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101286 DSG2 Fig.1 SDS PAGE 1

ECD Proteins

Human DSG2(50-609) Protein, His Tag

SKU:  PME101286     Target:  DSG2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101285 FGF19 Fig.1 SDS PAGE 1

ECD Proteins

Human FGF19 Protein, His Tag

SKU:  PME101285     Target:  FGF19     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101284 PTGFRN Fig.1 SDS PAGE 1

ECD Proteins

Human PTGFRN Protein, His Tag

SKU:  PME101284     Target:  PTGFRN     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101283 CD300LF Fig.1 SDS PAGE 1

ECD Proteins

Human CD300LF Protein, hFc Tag

SKU:  PME101283     Target:  CD300LF     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101282 CHRM2 Fig.1 SDS PAGE 1

ECD Proteins

Human CHRM2 Protein, hFc Tag

SKU:  PME101282     Target:  CHRM2     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101281 PF4V1 Fig.1 SDS PAGE 1

ECD Proteins

Human PF4V1 Protein, hFc Tag

SKU:  PME101281     Target:  PF4V1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme100730 cd3d cd3e sp1

ECD Proteins

Human CD3D Protein, His Tag and Human CD3E Protein, hFc Tag

SKU:  PME100730     Target:  CD3D; CD3E     Tag:  C-6×His Tag and C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101305 CGRP Fig.1 SDS PAGE 1

ECD Proteins

Human CGRP Protein, hFc Tag

SKU:  PME101305     Target:  CGRP     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101293 CDH19 Fig.1 SDS PAGE 1

ECD Proteins

Human CDH19 Protein, His Tag

SKU:  PME101293     Target:  CDH19     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101290 FCGR3B Fig.1 SDS PAGE 1

ECD Proteins

Human FCGR3B Protein, His Tag

SKU:  PME101290     Target:  FCGR3B     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101280 ECSCR Fig.1 SDS PAGE 1

ECD Proteins

Human ECSCR Protein, hFc Tag

SKU:  PME101280     Target:  ECSCR     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101279 TAFA5 Fig.1 SDS PAGE 1

ECD Proteins

Human TAFA5 Protein, hFc Tag

SKU:  PME101279     Target:  TAFA5     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101278 FCGR3B Fig.1 SDS PAGE 1

ECD Proteins

Human FCGR3B Protein, hFc Tag

SKU:  PME101278     Target:  FCGR3B     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101277 GPR75 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR75 Protein, mFc Tag

SKU:  PME101277     Target:  GPR75     Tag:  C-Mouse Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101276 CPM Fig.1 SDS PAGE 1

ECD Proteins

Human CPM Protein, His Tag

SKU:  PME101276     Target:  CPM     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101275 MANSC1 Fig.1 SDS PAGE 1

ECD Proteins

Human MANSC1 Protein, His Tag

SKU:  PME101275     Target:  MANSC1     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101274 PILRA Fig.1 SDS PAGE 1

ECD Proteins

Human PILRA Protein, hFc Tag

SKU:  PME101274     Target:  PILRA     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101273 GPR20 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR20 Protein, hFc Tag

SKU:  PME101273     Target:  GPR20     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101272 HAMP Fig.1 SDS PAGE 1

ECD Proteins

Human HAMP(60-84) Protein, hFc Tag

SKU:  PME101272     Target:  HAMP     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00