Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-PME101234 ACVRL1 Fig.1 SDS PAGE 1

ECD Proteins

Human ACVRL1 Protein, hFc Tag

SKU:  PME101234     Target:  ACVRL1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101233 ACVR1C Fig.1 SDS PAGE 1

ECD Proteins

Human ACVR1C Protein, hFc Tag

SKU:  PME101233     Target:  ACVR1C     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101232 LYPD3 Fig.1 SDS PAGE 1

ECD Proteins

Human LYPD3(31-325) Protein, His Tag

SKU:  PME101232     Target:  LYPD3     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101231 LYPD3 Fig.1 SDS PAGE 1

ECD Proteins

Human LYPD3(31-286) Protein, His Tag

SKU:  PME101231     Target:  LYPD3     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101230 CD14 Fig.1 SDS PAGE 1

ECD Proteins

Human CD14(20-352) Protein, His Tag

SKU:  PME101230     Target:  CD14     Tag:  C-7×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101229 CD14 Fig.1 SDS PAGE 1

ECD Proteins

Human CD14(20-344) Protein, His Tag

SKU:  PME101229     Target:  CD14     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101228 GPR56 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR56 Protein, His Tag

SKU:  PME101228     Target:  GPR56     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101227 ACHE Fig.1 SDS PAGE 1

ECD Proteins

Human ACHE Protein, His Tag

SKU:  PME101227     Target:  ACHE     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101226 ANGPTL7 Fig.1 SDS PAGE 1

ECD Proteins

Human ANGPTL7 Protein, His Tag

SKU:  PME101226     Target:  ANGPTL7     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101225 AZU1 Fig.1 SDS PAGE 1

ECD Proteins

Human AZU1 Protein, His Tag

SKU:  PME101225     Target:  AZU1     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101224 SEZ6L2 Fig.1 SDS PAGE 1

ECD Proteins

Human SEZ6L2 Protein, His Tag

SKU:  PME101224     Target:  SEZ6L2     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-PME101207 CLDN6 Fig.1 SDS PAGE 1

ECD Proteins

Human CLDN6(138-160) Protein, hFc Tag

SKU:  PME101207     Target:  CLDN6     Tag:  N-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101206 CLDN6 Fig.1 SDS PAGE 1

ECD Proteins

Human CLDN6(29-81) Protein, hFc Tag

SKU:  PME101206     Target:  CLDN6     Tag:  N-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-PME101255 GPNMB Fig.1 SDS PAGE 1

ECD Proteins

Human GPNMB Protein, His Tag

SKU:  PME101255     Target:  GPNMB     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-PME101252 LAIR1 Fig.1 SDS PAGE 1

ECD Proteins

Human LAIR1 Protein, hFc Tag

SKU:  PME101252     Target:  LAIR1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

PME101246 human CXADR hFc recombinant protein

ECD Proteins

Human CXADR Protein, hFc Tag

SKU:  PME101246     Target:  CXADR     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101242 alpp sp1

ECD Proteins

Human ALPP Protein, His Tag

SKU:  PME101242     Target:  ALPP     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-pme101238 il23a il12b sp1

ECD Proteins

Human IL23A and IL12B Heterodimer Protein, hFc Tag and His Tag

SKU:  PME101238     Target:  IL23A and IL12B     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101223 siglec5 sp1

ECD Proteins

Human SIGLEC5 Protein, His Tag

SKU:  PME101223     Target:  SIGLEC5     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101222 sirpg sp1

ECD Proteins

Human SIRPG Protein, His Tag

SKU:  PME101222     Target:  SIRPG     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101221 spa17 sp1

ECD Proteins

Human SPA17 Protein, His Tag

SKU:  PME101221     Target:  SPA17     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101220 plxdc2 sp1

ECD Proteins

Human PLXDC2 Protein, His Tag

SKU:  PME101220     Target:  PLXDC2     Tag:  C-6×His Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101219 tff1 sp1

ECD Proteins

Human TFF1 Protein, hFc Tag

SKU:  PME101219     Target:  TFF1     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101218 s100a4 sp1

ECD Proteins

Human S100A4 Protein, hFc Tag

SKU:  PME101218     Target:  S100A4     Tag:  C-Human Fc Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-pme101217 bmpr1a sp1

ECD Proteins

Human BMPR1A Protein, hFc Tag

SKU:  PME101217     Target:  BMPR1A     Tag:  C-Human Fc Tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101216 bmp2 sp1

ECD Proteins

Human BMP2 Protein, hFc Tag

SKU:  PME101216     Target:  BMP2     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101215 selenop sp1

ECD Proteins

Human Selenop Protein, Flag Tag

SKU:  PME101215     Target:  Selenop     Tag:  C-3×Flag Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101214 bace1 sp1

ECD Proteins

Human BACE1 Protein, His Tag

SKU:  PME101214     Target:  BACE1     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101213 bche sp1

ECD Proteins

Human BCHE Protein, His Tag

SKU:  PME101213     Target:  BCHE     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101212 angpt2 sp1

ECD Proteins

Human ANGPT2(275-496) Protein, His Tag

SKU:  PME101212     Target:  ANGPT2     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-pme101211 angpt2 sp1

ECD Proteins

Human ANGPT2(19-496) Protein, His Tag

SKU:  PME101211     Target:  ANGPT2     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101209 btn2a1 sp1

ECD Proteins

Human BTN2A1 Protein, His Tag

SKU:  PME101209     Target:  BTN2A1     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00