Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101174 msmp sp1

ECD Proteins

Human MSMP Protein, hFc Tag

SKU:  PME101174     Target:  MSMP     Tag:  C-Human Fc Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101168 CXCL8 Fig.1 SDS PAGE 1

ECD Proteins

Human IL8 Protein, His Tag

SKU:  PME101168     Target:  IL8     Tag:  C-6×His Tag

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101167 vps4 sp1

ECD Proteins

Human VPS4 Protein, His Tag

SKU:  PME101167     Target:  VPS4     Tag:  C-6×His Tag

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-pme101172 pdgfd sp1

ECD Proteins

Human PDGFD(250-370) Protein, hFc Tag

SKU:  PME101172     Target:  PDGFD     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101166 pdgfd sp1

ECD Proteins

Human PDGFD(19-370) Protein, His Tag

SKU:  PME101166     Target:  PDGFD     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101165 il17f sp1

ECD Proteins

Human IL17F Protein, His Tag

SKU:  PME101165     Target:  IL17F     Tag:  N-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101164 nrp2 sp1

ECD Proteins

Human NRP2 Protein, His Tag

SKU:  PME101164     Target:  NRP2     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101163 lrg1 sp1

ECD Proteins

Human LRG1 Protein, His Tag

SKU:  PME101163     Target:  LRG1     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101162 agtr1 sp1

ECD Proteins

Human AGTR1 Protein, hFc Tag

SKU:  PME101162     Target:  AGTR1     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101160 il10 sp1

ECD Proteins

Human IL10 Protein, His Tag

SKU:  PME101160     Target:  IL10     Tag:  N-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101159 fgl1 sp1

ECD Proteins

Human FGL1 Protein, His Tag

SKU:  PME101159     Target:  FGL1     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101157 pttg1ip sp1

ECD Proteins

Human PTTG1IP Protein, hFc Tag

SKU:  PME101157     Target:  PTTG1IP     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101156 il9 sp1

ECD Proteins

Human IL9 Protein, hFc Tag

SKU:  PME101156     Target:  IL9     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101155 il9 sp1

ECD Proteins

Human IL9 Protein, His Tag

SKU:  PME101155     Target:  IL9     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101154 ulbp2 sp1

ECD Proteins

Human ULBP2 Protein, His Tag

SKU:  PME101154     Target:  ULBP2     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101153 prtn3 sp1

ECD Proteins

Human PRTN3 Protein, His Tag

SKU:  PME101153     Target:  PRTN3     Tag:  C-6×His Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme30086 fgf 9 sp1

ECD Proteins

Human FGF-9 Protein

SKU:  PME30086     Target:  FGF-9     Tag: 

Price: 50 μg $400.00

sp-pme30085 tnf sp1

ECD Proteins

Human TNF beta Protein

SKU:  PME30085     Target:  TNF     Tag: 

Price: 50 μg $400.00

sp-pme30084 cntf sp1

ECD Proteins

Human CNTF Protein

SKU:  PME30084     Target:  CNTF     Tag: 

Price: 50 μg $400.00

sp-pme30083 ccl28 sp1

ECD Proteins

Human CCL28 Protein

SKU:  PME30083     Target:  CCL28     Tag: 

Price: 50 μg $400.00

sp-pme30082 ccl23 sp1

ECD Proteins

Human CCL23 Protein

SKU:  PME30082     Target:  CCL23     Tag: 

Price: 50 μg $400.00

sp-pme30081 ccl27 sp1

ECD Proteins

Human CCL27 Protein

SKU:  PME30081     Target:  CCL27     Tag: 

Price: 50 μg $400.00

sp-pme30079 ccl5 sp1

ECD Proteins

Human CCL5 Protein

SKU:  PME30079     Target:  CCL5     Tag: 

Price: 50 μg $400.00

sp-pme30078 ccl3 sp1

ECD Proteins

Human CCL3 Protein

SKU:  PME30078     Target:  CCL3     Tag: 

Price: 50 μg $400.00

sp-pme30077 cxcl14 sp1

ECD Proteins

Human CXCL14 Protein

SKU:  PME30077     Target:  CXCL14     Tag: 

Price: 50 μg $400.00

sp-pme30076 cxcl6 sp1

ECD Proteins

Human CXCL6 (C-6His) Protein

SKU:  PME30076     Target:  CXCL6     Tag: 

Price: 50 μg $377.00

sp-pme30075 igfbp 1 sp1

ECD Proteins

Human IGFBP-1 (C-6His) Protein

SKU:  PME30075     Target:  IGFBP-1     Tag:  C-6×His Tag

Price: 50 μg $360.00

sp-pme30074 il 28a sp1

ECD Proteins

Human IL-28A (C-6His) Protein

SKU:  PME30074     Target:  IL-28A     Tag:  C-6×His Tag

Price: 50 μg $360.00

sp-pme30073 il 28b sp1

ECD Proteins

Human IL-28B Protein

SKU:  PME30073     Target:  IL-28B     Tag: 

Price: 50 μg $360.00

sp-pme30072 il 15ra il 15 sp1

ECD Proteins

Human IL-15RAandIL-15 (C-Fc) Protein

SKU:  PME30072     Target:  IL-15RA and IL-15     Tag:  C-Fc Tag

Price: 50 μg $360.00

sp-pme30071 il 22ra2 sp1

ECD Proteins

Human IL-22RA2 (C-Fc) Protein

SKU:  PME30071     Target:  IL-22RA2     Tag:  C-Fc Tag

Price: 50 μg $360.00

sp-pme30070 ccl14 sp1

ECD Proteins

Human CCL14 (C-6His) Protein

SKU:  PME30070     Target:  CCL14     Tag:  C-6×His Tag

Price: 50 μg $360.00