Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

Blog

Multi-Species Recombinant Proteins: The Secret Weapon in Antibody Drug Discovery

Explore how recombinant proteins derived from human, mouse, monkey, and dog enable more accurate antigen selection, cross-species validation, and improved...

16
Nov
Domain-Specific Protein Fragments in Therapeutic Antibody Discovery

Using multiple recombinant protein fragments from the same target—such as extracellular domains, functional regions, and membrane-proximal segments—has become an essential...

08
Dec

The List of ECD Proteins

sp-pme m100018 hbegf sp1

ECD Proteins

Mouse HBEGF (24-160) Protein, hFc Tag

SKU:  PME-M100018     Target:  HBEGF     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100684 vwf sp1

ECD Proteins

Human VWF (764-2813) Protein, His Tag

SKU:  PME100684     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100683 vwf sp1

ECD Proteins

Human VWF (23-763) Protein, His Tag

SKU:  PME100683     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100682 ifnb1 sp1

ECD Proteins

Human IFNB1 Protein, hFc Tag

SKU:  PME100682     Target:  IFNB1     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100681 igfbp7 sp1

ECD Proteins

Human IGFBP7 Protein, His Tag

SKU:  PME100681     Target:  IGFBP7     Tag:  N-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100680 cd93 sp1

ECD Proteins

Human CD93 Protein, His Tag

SKU:  PME100680     Target:  CD93     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100679 rnase4 sp1

ECD Proteins

Human RNASE4 Protein, hFc Tag

SKU:  PME100679     Target:  RNASE4     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100678 rnase4 sp1

ECD Proteins

Human RNASE4 Protein, His Tag

SKU:  PME100678     Target:  RNASE4     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme m100019 cd30 sp1

ECD Proteins

Mouse CD30 Protein, hFc Tag

SKU:  PME-M100019     Target:  CD30     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100113 gpc3 sp1

ECD Proteins

Human GPC3 Protein, hFc Tag

SKU:  PME100113     Target:  GPC3     Tag:  N-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100646 notch3 sp1

ECD Proteins

Human NOTCH3 Protein, hFc Tag

SKU:  PME100646     Target:  NOTCH3     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme m100013 trop2 sp1

ECD Proteins

Mouse Trop2 Protein, hFc Tag

SKU:  PME-M100013     Target:  Trop2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100012 cd47 sp1

ECD Proteins

Mouse CD47 Protein, hFc Tag

SKU:  PME-M100012     Target:  CD47     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100011 cd27 sp1

ECD Proteins

Mouse CD27 Protein, hFc Tag

SKU:  PME-M100011     Target:  CD27     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme m100010 ctla4 sp1

ECD Proteins

Mouse CTLA4 Protein, hFc Tag

SKU:  PME-M100010     Target:  CTLA4     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100009 cd28 sp1

ECD Proteins

Mouse CD28 Protein, hFc Tag

SKU:  PME-M100009     Target:  CD28     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100008 b7 h3 sp1

ECD Proteins

Mouse B7-H3 Protein, hFc Tag

SKU:  PME-M100008     Target:  B7-H3     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100007 4 1bb sp1

ECD Proteins

Mouse 4-1BB Protein, hFc Tag

SKU:  PME-M100007     Target:  4-1BB     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100006 adam9 sp1

ECD Proteins

Mouse ADAM9 Protein, His Tag

SKU:  PME-M100006     Target:  ADAM9     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $450.00

sp-pme m100005 cd38 sp1

ECD Proteins

Mouse CD38 Protein, His Tag

SKU:  PME-M100005     Target:  CD38     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

100μg 50% 0FF
100μg 50% 0FF
sp-pme m100004 2b4 sp1

ECD Proteins

Mouse 2B4 Protein, hFc Tag

SKU:  PME-M100004     Target:  2B4     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100003 cd123 sp1

ECD Proteins

Mouse CD123 Protein, His Tag

SKU:  PME-M100003     Target:  CD123     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme m100002 cs1 sp1

ECD Proteins

Mouse CS1 Protein, hFc Tag

SKU:  PME-M100002     Target:  CS1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100001 bcma sp1

ECD Proteins

Mouse BCMA Protein, hFc Tag

SKU:  PME-M100001     Target:  BCMA     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100676 slc4a7 sp1

ECD Proteins

Human SLC4A7 Protein, His Tag

SKU:  PME100676     Target:  SLC4A7     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100675 mmp14 sp1

ECD Proteins

Human MMP14 Protein, hFc Tag

SKU:  PME100675     Target:  MMP14     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100674 cdh1 sp1

ECD Proteins

Human CDH1 Protein, hFc Tag

SKU:  PME100674     Target:  CDH1     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100673 cd32a sp1

ECD Proteins

Human CD32A (H167) Protein, hFc Tag

SKU:  PME100673     Target:  CD32a     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100668 gpr77 sp1

ECD Proteins

Human GPR77 (1-38) Protein, hFc Tag

SKU:  PME100668     Target:  C5AR2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100666 gfap sp1

ECD Proteins

Human GFAP (7-66) Protein, hFc Tag

SKU:  PME100666     Target:  GFAP     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100665 her2 sp1

ECD Proteins

Human Her2 Protein, hFc Tag

SKU:  PME100665     Target:  Her2     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100664 kcnk9 sp1

ECD Proteins

Human KCNK9 Protein, hFc Tag

SKU:  PME100664     Target:  KCNK9     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00