Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100663 slc2a4 sp1

ECD Proteins

Human SLC2A4 Protein, hFc Tag

SKU:  PME100663     Target:  SLC2A4     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100662 gpr87 sp1

ECD Proteins

Human GPR87 Protein, hFc Tag

SKU:  PME100662     Target:  GPR87     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100660 ccr6 sp1

ECD Proteins

Human CCR6 Protein, hFc Tag

SKU:  PME100660     Target:  CCR6     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100659 nefl sp1

ECD Proteins

Human NEFL Protein, hFc Tag

SKU:  PME100659     Target:  NEFL     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100687 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Omicron) S protein RBD, hFc Tag

SKU:  PME100687     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100658 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Delta) S protein RBD , hFc Tag

SKU:  PME100658     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100656 cd164 sp1

ECD Proteins

Human CD164 Protein, hFc Tag

SKU:  PME100656     Target:  CD164     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100654 nefl sp1

ECD Proteins

Human NEFL (9-88) Protein, hFc Tag

SKU:  PME100654     Target:  NEFL     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100653 grp sp1

ECD Proteins

Human GRP Protein, hFc Tag

SKU:  PME100653     Target:  GRP     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100612 eva1c sp1

ECD Proteins

Human EVA1C Protein, hFc Tag

SKU:  PME100612     Target:  EVA1C     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100650 fzd10 sp1

ECD Proteins

Human FZD10 Protein, hFc Tag

SKU:  PME100650     Target:  FZD10     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100651 il22 sp1

ECD Proteins

Human IL22 Protein, hFc Tag

SKU:  PME100651     Target:  IL22     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100645 siglec7 sp1

ECD Proteins

Human SIGLEC7 Protein, His Tag

SKU:  PME100645     Target:  SIGLEC7     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100644 cd23 sp1

ECD Proteins

Human CD23 Protein, His Tag

SKU:  PME100644     Target:  CD23     Tag:  N-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100643 cd205 sp1

ECD Proteins

Human CD205 Protein, His Tag

SKU:  PME100643     Target:  CD205     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100322 kras sp1

ECD Proteins

Human KRAS Protein, mFc Tag

SKU:  PME100322     Target:  KRAS     Tag:  C-mouse Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100901 adam9 sp1

ECD Proteins

Human ADAM9 Protein, His Tag

SKU:  PME100901     Target:  ADAM9     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100642 cd74 sp1

ECD Proteins

Human CD74 (73-296) Protein, hFc Tag

SKU:  PME100642     Target:  CD74     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100641 cd74 sp1

ECD Proteins

Human CD74 (73-232) Protein, hFc Tag

SKU:  PME100641     Target:  CD74     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100640 bst1 sp1

ECD Proteins

Human BST1 Protein, hFc Tag

SKU:  PME100640     Target:  BST1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100639 il11 sp1

ECD Proteins

Human IL11 Protein, hFc Tag

SKU:  PME100639     Target:  IL11     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100638 ghr sp1

ECD Proteins

Human GHR Protein, hFc Tag

SKU:  PME100638     Target:  GHR     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100637 tslp sp1

ECD Proteins

Human TSLP Protein, hFc Tag

SKU:  PME100637     Target:  TSLP     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100636 gp6 sp1

ECD Proteins

Human GP6 Protein, hFc Tag

SKU:  PME100636     Target:  GP6     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100635 apcdd1 sp1

ECD Proteins

Human APCDD1 Protein, His Tag

SKU:  PME100635     Target:  APCDD1     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100632 gp1bb sp1

ECD Proteins

Human GP1BB Protein, hFc Tag

SKU:  PME100632     Target:  GP1BB     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100631 ephb2 sp1

ECD Proteins

Human EPHB2 Protein, His Tag

SKU:  PME100631     Target:  EPHB2     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100625 fcrl5 sp1

ECD Proteins

Human FCRL5 Protein, His Tag

SKU:  PME100625     Target:  FCRL5     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100624 epha4 sp1

ECD Proteins

Human EPHA4 Protein, His Tag

SKU:  PME100624     Target:  EPHA4     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100621 gfral sp1

ECD Proteins

Human GFRAL Protein, His Tag

SKU:  PME100621     Target:  GFRAL     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100630 ror1 sp1

ECD Proteins

Human ROR1 (312-391) Protein, hFc Tag

SKU:  PME100630     Target:  ROR1     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100629 uchl1 sp1

ECD Proteins

Human UCHL1 Protein, hFc Tag

SKU:  PME100629     Target:  UCHL1     Tag:  N-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00