Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100628 gdf15 sp1

ECD Proteins

Human GDF15 Protein, hFc Tag

SKU:  PME100628     Target:  GDF15     Tag:  N-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100627 il17c sp1

ECD Proteins

Human IL17C Protein, hFc Tag

SKU:  PME100627     Target:  IL17C     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-PME100626 FAP His PAGE 300 1

ECD Proteins

Human FAP (30-760) Protein, His Tag

SKU:  PME100626     Target:  FAP     Tag:  N-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100622 adgre1 sp1

ECD Proteins

Human ADGRE1 Protein, His Tag

SKU:  PME100622     Target:  ADGRE1     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100620 gfra3 sp1

ECD Proteins

Human GFRA3 Protein, His Tag

SKU:  PME100620     Target:  GFRA3     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100634 mmp9 sp1

ECD Proteins

Human MMP9(107-707) Protein, His Tag

SKU:  PME100634     Target:  MMP9     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100618 fzd4 sp1

ECD Proteins

Human FZD4 Protein, hFc Tag

SKU:  PME100618     Target:  FZD4     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100617 ereg sp1

ECD Proteins

Human EREG Protein, hFc Tag

SKU:  PME100617     Target:  EREG     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101042 dkk2 sp1

ECD Proteins

Human DKK2 Protein, hFc Tag

SKU:  PME101042     Target:  DKK2     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-PME100849 CD9 Fig.1 SDS PAGE 1

ECD Proteins

Human CD9 Protein, His Tag

SKU:  PME100849     Target:  CD9     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100613 efna3 sp1

ECD Proteins

Human EFNA3 Protein, hFc Tag

SKU:  PME100613     Target:  EFNA3     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100611 cxcl4 sp1

ECD Proteins

Human CXCL4 Protein, hFc Tag

SKU:  PME100611     Target:  CXCL4     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100610 clec9a sp1

ECD Proteins

Human CLEC9A Protein, hFc Tag

SKU:  PME100610     Target:  CLEC9A     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-PME100609 CLEC1A hFc PAGE 300

ECD Proteins

Human CLEC1A Protein, hFc Tag

SKU:  PME100609     Target:  CLEC1A     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-PME100608 CHODL hFc PAGE 300

ECD Proteins

Human CHODL Protein, hFc Tag

SKU:  PME100608     Target:  CHODL     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100607 dll3 sp1 1

ECD Proteins

Human DLL3 Protein, hFc Tag

SKU:  PME100607     Target:  DLL3     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100606 cd69 sp1 1

ECD Proteins

Human CD69 Protein, hFc Tag

SKU:  PME100606     Target:  CD69     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100605 crtam sp1

ECD Proteins

Human CRTAM Protein, His Tag

SKU:  PME100605     Target:  CRTAM     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100615 epha5 sp1

ECD Proteins

Human EPHA5 Protein, His Tag

SKU:  PME100615     Target:  EPHA5     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100616 antxr1 sp1

ECD Proteins

Human ANTXR1 Protein, His Tag

SKU:  PME100616     Target:  ANTXR1     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100601 mdr 1 sp1

ECD Proteins

Human MDR-1 (72-113) Protein, hFc Tag

SKU:  PME100601     Target:  MDR-1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100600 cd68 sp1

ECD Proteins

Human CD68 Protein, His Tag

SKU:  PME100600     Target:  CD68     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100599 cd5l sp1

ECD Proteins

Human CD5L Protein, His Tag

SKU:  PME100599     Target:  CD5L     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100598 upa sp1

ECD Proteins

Human UPA Protein, His Tag

SKU:  PME100598     Target:  UPA     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100450 tyro3 sp1

ECD Proteins

Human TYRO3 Protein, His Tag

SKU:  PME100450     Target:  TYRO3     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100350 mif sp1

ECD Proteins

Human MIF Protein, hFc Tag

SKU:  PME100350     Target:  MIF     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100262 gucy2c sp1

ECD Proteins

Human GUCY2C Protein, His Tag

SKU:  PME100262     Target:  GUCY2C     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101053 il1a sp1

ECD Proteins

Human IL1A Protein, hFc Tag

SKU:  PME101053     Target:  IL1A     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100972 lgals3 sp1

ECD Proteins

Human LGALS3 Protein, His Tag

SKU:  PME100972     Target:  LGALS3     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100874 nectin 4 sp1 1

ECD Proteins

Human Nectin-4 Protein, His Tag

SKU:  PME100874     Target:  Nectin-4     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100597 cd62l sp1

ECD Proteins

Human CD62L Protein, His Tag

SKU:  PME100597     Target:  CD62L     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100596 cd83 sp1

ECD Proteins

Human CD83 Protein, hFc Tag

SKU:  PME100596     Target:  CD83     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00