Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100535 il6r sp1

ECD Proteins

Human IL6R Protein, hFc Tag

SKU:  PME100535     Target:  IL6R     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100053 b7 h4 sp1

ECD Proteins

Human B7-H4 Protein, hFc Tag

SKU:  PME100053     Target:  B7-H4     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100049 light sp1

ECD Proteins

Human LIGHT Protein, hFc Tag

SKU:  PME100049     Target:  LIGHT     Tag:  N-Human Fc Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-PME100530 CD38 Fig.1 SDS PAGE 1

ECD Proteins

Human CD38 Protein, hFc Tag

SKU:  PME100530     Target:  CD38     Tag:  C-Human Fc tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100526 btla sp1

ECD Proteins

Mouse BTLA Protein, His Tag

SKU:  PME100526     Target:  BTLA     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100524 btla sp1

ECD Proteins

Human BTLA Protein, His Tag

SKU:  PME100524     Target:  BTLA     Tag:  C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100523 btla sp1

ECD Proteins

Human BTLA Protein, hFc Tag

SKU:  PME100523     Target:  BTLA     Tag:  C-Human Fc Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-PME100538 TM4SF1 hFc PAGE 300

ECD Proteins

Human TM4SF1 Protein, hFc Tag

SKU:  PME100538     Target:  TM4SF1     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-PME100533 AXL mFc PAGE 300

ECD Proteins

Human AXL Protein, mFc Tag

SKU:  PME100533     Target:  AXL     Tag:  C-Mouse Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100532 axl sp1

ECD Proteins

Human AXL Protein, hFc Tag

SKU:  PME100532     Target:  AXL     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100531 sirpa sp1

ECD Proteins

Human SIRPa Protein, mFc Tag

SKU:  PME100531     Target:  SIRPa     Tag:  C-Mouse Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100046 cd20 sp1

ECD Proteins

Human CD20 Protein, hFc Tag

SKU:  PME100046     Target:  CD20     Tag:  N-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100377 p2rx7 sp1

ECD Proteins

Human P2RX7 Protein, hFc Tag

SKU:  PME100377     Target:  P2RX7     Tag:  N-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100525 btla sp1

ECD Proteins

Mouse BTLA Protein, mFc Tag

SKU:  PME100525     Target:  BTLA     Tag:  C-Mouse Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100520 masp2 sp1

ECD Proteins

Human MASP2 Protein, hFc Tag

SKU:  PME100520     Target:  MASP2     Tag:  N-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100539 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Beta) S protein RBD, hFc Tag

SKU:  PME100539     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100529 fap sp1

ECD Proteins

Mouse FAP Protein, C-mFc Tag

SKU:  PME100529     Target:  FAP     Tag:  C-Mouse Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100528 fap sp1

ECD Proteins

Mouse FAP Protein, N-mFc Tag

SKU:  PME100528     Target:  FAP     Tag:  N-Mouse Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100527 angptl3 sp1

ECD Proteins

Human ANGPTL3 Protein, His Tag

SKU:  PME100527     Target:  ANGPTL3     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100519 cd117 sp1

ECD Proteins

Human CD117 Protein, His Tag

SKU:  PME100519     Target:  CD117     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100518 pdl2 sp1

ECD Proteins

Human PDL2 Protein, His Tag

SKU:  PME100518     Target:  PDL2     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

fc-pme100517 cd160 fc1

ECD Proteins

Human CD160 Protein, His Tag

SKU:  PME100517     Target:  CD160     Tag:  C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100291 il13ra1 sp1

ECD Proteins

Human IL13RA1 Protein, His Tag

SKU:  PME100291     Target:  IL13RA1     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100104 vegfr2 sp1

ECD Proteins

Human VEGFR2 Protein, His Tag

SKU:  PME100104     Target:  VEGFR2     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100090 pvrig sp1

ECD Proteins

Human PVRIG Protein, hFc Tag

SKU:  PME100090     Target:  PVRIG     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100055 cd160 sp1

ECD Proteins

Human CD160 Protein, hFc Tag

SKU:  PME100055     Target:  CD160     Tag:  C-Human Fc Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100516 micb sp1

ECD Proteins

Human MICB Protein, His Tag

SKU:  PME100516     Target:  MICB     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100515 galectin 9 sp1

ECD Proteins

Human Galectin 9 Protein, hFc Tag

SKU:  PME100515     Target:  Galectin 9     Tag:  N-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100513 lag3 sp1

ECD Proteins

Human LAG3 Protein, hFc Tag

SKU:  PME100513     Target:  LAG3     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100512 lag3 sp1

ECD Proteins

Human LAG3 Protein, His Tag

SKU:  PME100512     Target:  LAG3     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100511 bcma sp1

ECD Proteins

Human BCMA Protein, His Tag

SKU:  PME100511     Target:  BCMA     Tag:  C-6×His Tag

Price: 10μg $91.00; 50μg $348.00 ; 100 μg $522.00

sp-pme100349 mica sp1

ECD Proteins

Human MICA Protein, His Tag

SKU:  PME100349     Target:  MICA     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00