Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100561 gprc5d sp1

ECD Proteins

Cynomolgus GPRC5D (1-21) Protein, hFc Tag

SKU:  PME100561     Target:  GPRC5D     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100554 jam a sp1

ECD Proteins

Human JAM-A Protein, hFc Tag

SKU:  PME100554     Target:  JAM-A     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100553 cd112 sp1

ECD Proteins

Human CD112 Protein, hFc Tag

SKU:  PME100553     Target:  CD112     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100552 il4ra sp1

ECD Proteins

Human IL4RA Protein, His Tag

SKU:  PME100552     Target:  IL4RA     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100551 il4ra sp1

ECD Proteins

Human IL4RA Protein, mFc Tag

SKU:  PME100551     Target:  IL4RA     Tag:  C-Mouse Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100550 m csf sp1

ECD Proteins

Human M-CSF Protein, hFc Tag

SKU:  PME100550     Target:  M-CSF     Tag:  C-Human Fc Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100300 il2 sp1

ECD Proteins

Human IL2 Protein, hFc Tag

SKU:  PME100300     Target:  IL2     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100187 cd36 sp1

ECD Proteins

Human CD36 Protein, hFc Tag

SKU:  PME100187     Target:  CD36     Tag:  C-Human Fc Tag

Price: 10μg $91.00; 50μg $328.00 ; 100μg $488.00

sp-pme100159 ccl20 sp1

ECD Proteins

Human CCL20 Protein, hFc Tag

SKU:  PME100159     Target:  CCL20     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100549 amhr2 sp1

ECD Proteins

Human AMHR2 Protein, hFc Tag

SKU:  PME100549     Target:  AMHR2     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100548 il15ra sp1

ECD Proteins

Human IL15RA Protein, hFc Tag

SKU:  PME100548     Target:  IL15RA     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100230 dkk1 sp1

ECD Proteins

Human DKK1 Protein, hFc Tag

SKU:  PME100230     Target:  DKK1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100547 muc1 sp1

ECD Proteins

Human MUC1 Protein, hFc Tag

SKU:  PME100547     Target:  MUC1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100546 psma sp1

ECD Proteins

Human PSMA Protein, mFc Tag

SKU:  PME100546     Target:  PSMA     Tag:  N-Mouse Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100545 psma sp1

ECD Proteins

Human PSMA Protein, His Tag

SKU:  PME100545     Target:  PSMA     Tag:  N-6×His Tag

Price: 10μg $137.00 ; 50μg $513.00 ; 100 μg $752.00

sp-pme100544 yap1 sp1

ECD Proteins

Human YAP1 Protein, His Tag

SKU:  PME100544     Target:  YAP1     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100697 ncl sp1

ECD Proteins

Human NCL Protein, His Tag

SKU:  PME100697     Target:  NCL     Tag:  N-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100543 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Alpha) S protein RBD , hFc Tag

SKU:  PME100543     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100534 sfrp2 sp1

ECD Proteins

Human SFRP2 Protein, His Tag

SKU:  PME100534     Target:  SFRP2     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100390 psma sp1

ECD Proteins

Human PSMA Protein, hFc Tag

SKU:  PME100390     Target:  PSMA     Tag:  N-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100301 il21r sp1

ECD Proteins

Human IL21R Protein, hFc Tag

SKU:  PME100301     Target:  IL21R     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100542 tmem173 sp1

ECD Proteins

Human TMEM173 Protein, hFc Tag

SKU:  PME100542     Target:  TMEM173     Tag:  N-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100541 slamf3 sp1

ECD Proteins

Human SLAMF3 Protein, His Tag

SKU:  PME100541     Target:  SLAMF3     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100086 ptk7 sp1

ECD Proteins

Human PTK7 Protein, hFc Tag

SKU:  PME100086     Target:  PTK7     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100064 tgfb1 sp1

ECD Proteins

Human TGFB1 Protein, hFc Tag

SKU:  PME100064     Target:  TGFB1     Tag:  N-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100057 slamf3 sp1

ECD Proteins

Human SLAMF3 Protein, hFc Tag

SKU:  PME100057     Target:  SLAMF3     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100540 cd56 sp1

ECD Proteins

Human CD56 Protein, hFc Tag

SKU:  PME100540     Target:  CD56     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100249 folr1 sp1

ECD Proteins

Human FOLR1 Protein, His Tag

SKU:  PME100249     Target:  FOLR1     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100194 cd56 sp1

ECD Proteins

Human CD56 Protein, His Tag

SKU:  PME100194     Target:  CD56     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100075 afp sp1

ECD Proteins

Human AFP Protein, hFc Tag

SKU:  PME100075     Target:  AFP     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100537 il5 sp1

ECD Proteins

Human IL5 Protein, hFc Tag

SKU:  PME100537     Target:  IL5     Tag:  C-Human Fc Tag

Price: 10μg $96.00; 50μg $349.00 ; 100μg $520.00

sp-pme100536 il5ra sp1

ECD Proteins

Human IL5RA Protein, hFc Tag

SKU:  PME100536     Target:  IL5RA     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00