Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100595 cd2 sp1

ECD Proteins

Human CD2 Protein, hFc Tag

SKU:  PME100595     Target:  CD2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100594 cd81 sp1

ECD Proteins

Human CD81 Protein, hFc Tag

SKU:  PME100594     Target:  CD81     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100582 cd26 sp1

ECD Proteins

Human CD26 Protein, His Tag

SKU:  PME100582     Target:  CD26     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100581 cd45 sp1

ECD Proteins

Human CD45 Protein, His Tag

SKU:  PME100581     Target:  CD45     Tag:  C-6×His Tag

Price: 10μg $91.00; 50μg $328.00 ; 100μg $488.00

sp-pme100579 pgf sp1

ECD Proteins

Human PGF Protein, hFc Tag

SKU:  PME100579     Target:  PGF     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100578 il11ra sp1

ECD Proteins

Human IL11RA Protein, hFc Tag

SKU:  PME100578     Target:  IL11RA     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100577 tlr3 sp1

ECD Proteins

Human TLR3 Protein, His Tag

SKU:  PME100577     Target:  TLR3     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100576 cd112 sp1

ECD Proteins

Human CD112 Protein, His Tag

SKU:  PME100576     Target:  CD112     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100575 cd112 sp1

ECD Proteins

Human CD112 Protein, mFc Tag

SKU:  PME100575     Target:  CD112     Tag:  C-Mouse Fc Tag

Price: 10μg $96.00; 50μg $349.00 ; 100μg $520.00

sp-pme100591 alb sp1

ECD Proteins

Human ALB Protein, His Tag

SKU:  PME100591     Target:  ALB     Tag:  C-6×His Tag

Price: 10μg $91.00; 50μg $328.00 ; 100μg $488.00

sp-pme100590 cd26 sp1

ECD Proteins

Human CD26 (29-766) Protein, His Tag

SKU:  PME100590     Target:  CD26     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100589 mdr 1 sp1

ECD Proteins

Human MDR-1 Protein, hFc Tag

SKU:  PME100589     Target:  MDR-1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100587 il21r sp1

ECD Proteins

Human IL21R Protein, His Tag

SKU:  PME100587     Target:  IL21R     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100851 nkg2a sp1

ECD Proteins

Human NKG2A Protein, hFc Tag

SKU:  PME100851     Target:  NKG2A     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100695 cd93 sp1

ECD Proteins

Human CD93 Protein, mFc Tag

SKU:  PME100695     Target:  CD93     Tag:  C-Mouse Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100572 slc7a11 sp1

ECD Proteins

Human SLC7A11 Protein, hFc Tag

SKU:  PME100572     Target:  SLC7A11     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100564 gm csf sp1

ECD Proteins

Human GM-CSF Protein, hFc Tag

SKU:  PME100564     Target:  GM-CSF     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100562 pcsk9 sp1

ECD Proteins

Human PCSK9 Protein, hFc Tag

SKU:  PME100562     Target:  PCSK9     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100134 alk sp1

ECD Proteins

Human ALK Protein, hFc Tag

SKU:  PME100134     Target:  ALK     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100584 cfb sp1

ECD Proteins

Human CFB Protein, hFc Tag

SKU:  PME100584     Target:  CFB     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100112 ccr4 sp1

ECD Proteins

Human CCR4 Protein, hFc Tag

SKU:  PME100112     Target:  CCR4     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100574 il2 sp1

ECD Proteins

Human IL2 Protein, mFc Tag

SKU:  PME100574     Target:  IL2     Tag:  C-Mouse Fc Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100571 ntrk1 sp1

ECD Proteins

Human NTRK1 Protein, hFc Tag

SKU:  PME100571     Target:  NTRK1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100570 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Kappa) nCoV S Protein RBD Protein, hFc Tag

SKU:  PME100570     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100569 m csf sp1

ECD Proteins

Human M-CSF Protein, His Tag

SKU:  PME100569     Target:  M-CSF     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100568 m csf sp1

ECD Proteins

Human M-CSF Protein, mFc Tag

SKU:  PME100568     Target:  M-CSF     Tag:  C-Mouse Fc Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100567 bcl2l1 sp1

ECD Proteins

Human BCL2L1 Protein, hFc Tag

SKU:  PME100567     Target:  BCL2L1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100566 ntrk3 sp1

ECD Proteins

Human NTRK3 Protein, hFc Tag

SKU:  PME100566     Target:  NTRK3     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100558 il18 sp1

ECD Proteins

Human IL18 Protein, mFc Tag

SKU:  PME100558     Target:  IL18     Tag:  C-Mouse Fc tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100429 tlr3 sp1

ECD Proteins

Human TLR3 Protein, mFc Tag

SKU:  PME100429     Target:  TLR3     Tag:  C-Mouse Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100130 adora2a sp1

ECD Proteins

Human ADORA2A Protein, hFc Tag

SKU:  PME100130     Target:  ADORA2A     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100563 scf sp1

ECD Proteins

Human SCF Protein, hFc Tag

SKU:  PME100563     Target:  SCF     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00