Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100238 epha2 sp1

ECD Proteins

Human EPHA2 Protein, His Tag

SKU:  PME100238     Target:  EPHA2     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100217 cspg4 sp1

ECD Proteins

Human CSPG4 Protein, His Tag

SKU:  PME100217     Target:  CSPG4     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100691 lama4 sp1

ECD Proteins

Human LAMA4 Protein, His Tag

SKU:  PME100691     Target:  LAMA4     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100115 pmel sp1

ECD Proteins

Human PMEL Protein, hFc Tag

SKU:  PME100115     Target:  PMEL     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100100 tnfsf11 sp1

ECD Proteins

Human TNFSF11 Protein, hFc Tag

SKU:  PME100100     Target:  TNFSF11     Tag:  N-Human Fc Tag

Price: 10μg $85.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100060 dll3 sp1

ECD Proteins

Human DLL3 Protein, His Tag

SKU:  PME100060     Target:  DLL3     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100111 vegfa sp1

ECD Proteins

Human VEGFA Protein, His Tag

SKU:  PME100111     Target:  VEGFA     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100108 cd44 sp1

ECD Proteins

Human CD44 Protein, His Tag

SKU:  PME100108     Target:  CD44     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100510 b7 h6 sp1

ECD Proteins

Human B7-H6 Protein, His Tag

SKU:  PME100510     Target:  B7-H6     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100509 cd10 sp1

ECD Proteins

Human CD10 Protein, hFc Tag

SKU:  PME100509     Target:  CD10     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100672 tim1 sp1

ECD Proteins

Human TIM1 Protein, His Tag

SKU:  PME100672     Target:  TIM1     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100508 cb1 sp1

ECD Proteins

Human CB1 Protein, hFc Tag

SKU:  PME100508     Target:  CB1     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100507 cb1 sp1

ECD Proteins

Human CB1 (1-116) Protein, hFc Tag

SKU:  PME100507     Target:  CB1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100490 ace2 sp1

ECD Proteins

Human ACE2 Protein, His Tag

SKU:  PME100490     Target:  ACE2     Tag:  N-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100273 hvem sp1

ECD Proteins

Human HVEM Protein, His Tag

SKU:  PME100273     Target:  HVEM     Tag:  C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100173 cd171 sp1

ECD Proteins

Human CD171 Protein, His Tag

SKU:  PME100173     Target:  CD171     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100152 ca9 sp1

ECD Proteins

Human CA9 Protein, His Tag

SKU:  PME100152     Target:  CA9     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100123 5t4 sp1

ECD Proteins

Human 5T4 Protein, His Tag

SKU:  PME100123     Target:  5T4     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100109 il6r sp1

ECD Proteins

Human IL6R Protein, His Tag

SKU:  PME100109     Target:  IL6R     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100106 muc1 sp1

ECD Proteins

Human MUC1 Protein, HisTag

SKU:  PME100106     Target:  MUC1     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100105 tnfsf12 sp1

ECD Proteins

Human TNFSF12 Protein, hFc Tag

SKU:  PME100105     Target:  TNFSF12     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100047 b7 1 sp1

ECD Proteins

Human B7-1 Protein, mFc-His Tag

SKU:  PME100047     Target:  B7-1     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

Human CD32a (R167) Protein, hFc Tag

ECD Proteins

Human CD32a (R167) Protein, hFc Tag

SKU:  PME101102     Target:  CD32a     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101101 zp3 sp1

ECD Proteins

Human ZP3 Protein, hFc Tag

SKU:  PME101101     Target:  ZP3     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101100 enpp3 sp1

ECD Proteins

Human ENPP3 Protein, hFc Tag

SKU:  PME101100     Target:  ENPP3     Tag:  N-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101099 scgb2a2 sp1

ECD Proteins

Human SCGB2A2 Protein, hFc Tag

SKU:  PME101099     Target:  SCGB2A2     Tag:  C-Human Fc tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-PME101098 ADAM17 Fig.1 SDS PAGE 1

ECD Proteins

Human ADAM17(581-642) Protein, hFc Tag

SKU:  PME101098     Target:  ADAM17     Tag:  C-Human Fc tag

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101093 nrg1 sp1

ECD Proteins

Human NRG1 Protein, hFc Tag

SKU:  PME101093     Target:  NRG1     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101092 hgf sp1

ECD Proteins

Human HGF Protein, hFc Tag

SKU:  PME101092     Target:  HGF     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101091 ccr8 sp1

ECD Proteins

Human CCR8 Protein, hFc Tag

SKU:  PME101091     Target:  CCR8     Tag:  C-Human Fc Tag

Price: 10μg $100.00; 50μg $366.00 ; 100 μg $549.00

sp-pme101089 cd79b sp1

ECD Proteins

Human CD79B Protein, hFc Tag

SKU:  PME101089     Target:  CD79B     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101088 il21 sp1

ECD Proteins

Human IL21 Protein, hFc Tag

SKU:  PME101088     Target:  IL21     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00