Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101085 cd43 sp1

ECD Proteins

Human CD43 Protein, hFc Tag

SKU:  PME101085     Target:  CD43     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101083 il20 sp1

ECD Proteins

Human IL20 Protein, hFc Tag

SKU:  PME101083     Target:  IL20     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101080 ccr3 sp1

ECD Proteins

Human CCR3 Protein, hFc Tag

SKU:  PME101080     Target:  CCR3     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101079 il18ra sp1

ECD Proteins

Human IL18RA Protein, hFc Tag

SKU:  PME101079     Target:  IL18RA     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101078 ct83 sp1

ECD Proteins

Human CT83 Protein, hFc Tag

SKU:  PME101078     Target:  CT83     Tag:  N-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101076 gpc1 sp1

ECD Proteins

Human GPC1 Protein, His Tag

SKU:  PME101076     Target:  GPC1     Tag:  C-7×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101075 cxcl16 sp1

ECD Proteins

Human CXCL16 Protein, hFc Tag

SKU:  PME101075     Target:  CXCL16     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101070 tm4sf1 sp1

ECD Proteins

Human TM4SF1 (31-49) Protein, hFc Tag

SKU:  PME101070     Target:  TM4SF1     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101068 il2rg sp1

ECD Proteins

Human IL2RG Protein, hFc Tag

SKU:  PME101068     Target:  IL2RG     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101067 ceacam8 sp1

ECD Proteins

Human CEACAM8 Protein, hFc Tag

SKU:  PME101067     Target:  CEACAM8     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101060 cxcl13 sp1

ECD Proteins

Human CXCL13 Protein, hFc Tag

SKU:  PME101060     Target:  CXCL13     Tag:  N-Human Fc Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101058 ifngr1 sp1

ECD Proteins

Human IFNGR1 Protein, hFc Tag

SKU:  PME101058     Target:  IFNGR1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101055 il18 sp1

ECD Proteins

Human IL18 Protein, hFc Tag

SKU:  PME101055     Target:  IL18     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100208 clec2d sp1

ECD Proteins

Human CLEC2D Protein, N-His Tag

SKU:  PME100208     Target:  CLEC2D     Tag:  N-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101051 fgl1 sp1

ECD Proteins

Human FGL1 Protein, hFc Tag

SKU:  PME101051     Target:  FGL1     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101050 cd24 sp1

ECD Proteins

Human CD24 Protein, hFc Tag

SKU:  PME101050     Target:  CD24     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101045 faslg sp1

ECD Proteins

Human FASLG Protein, hFc Tag

SKU:  PME101045     Target:  FASLG     Tag:  N-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100603 clec14a sp1

ECD Proteins

Human CLEC14A Protein, His Tag

SKU:  PME100603     Target:  CLEC14A     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100602 cdcp1 sp1

ECD Proteins

Human CDCP1 Protein, His Tag

SKU:  PME100602     Target:  CDCP1     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101041 b7 h5 sp1

ECD Proteins

Human B7-H5 Protein, hFc Tag

SKU:  PME101041     Target:  B7-H5     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101039 cd6 sp1

ECD Proteins

Human CD6 Protein, His Tag

SKU:  PME101039     Target:  CD6     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100557 il2 sp1

ECD Proteins

Human IL2 Protein, His Tag

SKU:  PME100557     Target:  IL2     Tag:  C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme101032 flt1 sp1

ECD Proteins

Human FLT1 Protein, hFc Tag

SKU:  PME101032     Target:  FLT1     Tag:  C-Human Fc Tag

Price: 10μg $137.00 ; 50μg $513.00 ; 100 μg $752.00

sp-pme101025 tnfrsf1a sp1

ECD Proteins

Human TNFRSF1A Protein, hFc Tag

SKU:  PME101025     Target:  TNFRSF1A     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100593 ptn sp1

ECD Proteins

Human PTN Protein, hFc Tag

SKU:  PME100593     Target:  PTN     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101020 tlr3 sp1

ECD Proteins

Human TLR3 Protein, hFc Tag

SKU:  PME101020     Target:  TLR3     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100592 fgfr4 sp1

ECD Proteins

Human FGFR4 Protein, His Tag

SKU:  PME100592     Target:  FGFR4     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101015 tgfb2 sp1

ECD Proteins

Human TGFB2(303-414) Protein, hFc Tag

SKU:  PME101015     Target:  TGFB2     Tag:  N-Human Fc tag

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101008 spam1 sp1

ECD Proteins

Human SPAM1 Protein, hFc Tag

SKU:  PME101008     Target:  SPAM1     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101006 siglec9 sp1

ECD Proteins

Human SIGLEC9 Protein, His Tag

SKU:  PME101006     Target:  SIGLEC9     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101003 robo1 sp1

ECD Proteins

Human ROBO1 Protein, hFc Tag

SKU:  PME101003     Target:  ROBO1     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101001 rhoc sp1

ECD Proteins

Human RHOC Protein, hFc Tag

SKU:  PME101001     Target:  RhoC     Tag:  C-Human Fc Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00