Extracellular Domain (ECD) Protein
Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.
Features and Applications
Features
Used as lmmunogen for
Therapeutic mAbs
Different Animal Model Species and Fragments
Validated in Therapeutic
mAbs Functional Screening
For Immunization
ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.
Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.
Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
For Protein Activity
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.
Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.
Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.
HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
Hot Druggable Targets
Immune checkpoint proteins
Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.
Hematological cancer targets
Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.
More hematological cancer
Solid tumor targets
Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.
More solid tumor targetsBlog
The List of ECD Proteins
ECD Proteins
SKU: PME30030 Target: Beta-NGF Tag:
Price: 50 μg $103.00
ECD Proteins
SKU: PME30026 Target: Vitronectin Tag: N-Truncated, C-6×His Tag
Price: 50 μg $103.00
ECD Proteins
SKU: PME30025 Target: KGF Tag: C-6×His Tag
Price: 50 μg $503.00
ECD Proteins
SKU: PME30023 Target: TPO Tag: N, C-6×His Tag
Price: 50 μg $503.00
ECD Proteins
SKU: PME30020 Target: IFN Tag: C-6×His Tag
Price: 50 μg $503.00
ECD Proteins
SKU: PME30019 Target: TNF Tag: C-6×His Tag
Price: 50 μg $503.00
ECD Proteins
SKU: PME30018 Target: CCL18 Tag: C-6×His Tag
Price: 50 μg $503.00
ECD Proteins
SKU: PME30017 Target: IRF5 Tag: C-6×His Tag
Price: 50 μg $503.00
ECD Proteins
SKU: PME30016 Target: CCL3L1 Tag: C-6×His Tag
Price: 50 μg $503.00
ECD Proteins
SKU: PME30014 Target: OSM Tag: N-6×His Tag
Price: 50 μg $503.00
ECD Proteins
SKU: PME30008 Target: M-CSF Tag: C-6×His Tag
Price: 50 μg $503.00
ECD Proteins
SKU: PME101161 Target: E8L Tag: C-6×His Tag
Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00