Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101195 vstm5 sp1

ECD Proteins

Human VSTM5 Protein, hFc Tag

SKU:  PME101195     Target:  VSTM5     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-pme101192 timp1 sp1

ECD Proteins

Human TIMP1 Protein, hFc Tag

SKU:  PME101192     Target:  TIMP1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101191 il23a sp1

ECD Proteins

Human IL23A Protein, hFc Tag

SKU:  PME101191     Target:  IL23A     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101190 hcrtr1 sp1

ECD Proteins

Human HCRTR1 Protein, hFc Tag

SKU:  PME101190     Target:  HCRTR1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101189 cldn6 sp1

ECD Proteins

Human CLDN6(138-160) Protein, mFc Tag

SKU:  PME101189     Target:  CLDN6     Tag:  C-mouse Fc Tag

Expression Host:  HEK293

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101188 cldn6 sp1

ECD Proteins

Human CLDN6(138-160) Protein, hFc Tag

SKU:  PME101188     Target:  CLDN6     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101177 podxl2 sp1

ECD Proteins

Human PODXL2 Protein, His Tag

SKU:  PME101177     Target:  PODXL2     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101171 cdh11 sp1

ECD Proteins

Human CDH11(23-617) Protein, His Tag

SKU:  PME101171     Target:  CDH11     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101169 cdh10 sp1

ECD Proteins

Human CDH10 Protein, His Tag

SKU:  PME101169     Target:  CDH10     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme100082 tnfrsf1b sp1

ECD Proteins

Human TNFRSF1B Protein, hFc Tag

SKU:  PME100082     Target:  TNFRSF1B     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101194 sez6 sp1

ECD Proteins

Human SEZ6 Protein, His Tag

SKU:  PME101194     Target:  SEZ6     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101170 cdh11 sp1

ECD Proteins

Human CDH11(54-617) Protein, His Tag

SKU:  PME101170     Target:  CDH11     Tag:  C-6×His Tag

Expression Host:  HEK293

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101193 lrp6 sp1

ECD Proteins

Human LRP6 Protein, His Tag

SKU:  PME101193     Target:  LRP6     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $91.00; 50μg $328.00 ; 100μg $488.00

sp-pme101187 lgals3bp sp1

ECD Proteins

Human LGALS3BP Protein, Flag Tag

SKU:  PME101187     Target:  LGALS3BP     Tag:  C-3×Flag Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101186 btk sp1

ECD Proteins

Human BTK Protein, Flag Tag

SKU:  PME101186     Target:  BTK     Tag:  C-3×Flag Tag

Expression Host:  HEK293

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-pme101184 cldn4 sp1

ECD Proteins

Human CLDN4(145-157) Protein, mFc Tag

SKU:  PME101184     Target:  CLDN4     Tag:  C-mouse Fc Tag

Expression Host:  HEK293

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-pme101183 cldn4 sp1

ECD Proteins

Human CLDN4(145-157) Protein, hFc Tag

SKU:  PME101183     Target:  CLDN4     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101182 cldn3 sp1

ECD Proteins

Human CLDN3(144-156) Protein, mFc Tag

SKU:  PME101182     Target:  CLDN3     Tag:  C-mouse Fc Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101181 cldn3 sp1

ECD Proteins

Human CLDN3(144-156) Protein, hFc Tag

SKU:  PME101181     Target:  CLDN3     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101180 fzd7 sp1

ECD Proteins

Human FZD7 Protein, hFc Tag

SKU:  PME101180     Target:  FZD7     Tag:  N-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101178 postn sp1

ECD Proteins

Human POSTN Protein, His Tag

SKU:  PME101178     Target:  POSTN     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101175 prokr1 sp1

ECD Proteins

Human PROKR1 Protein, hFc Tag

SKU:  PME101175     Target:  PROKR1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $70.00; 50μg $244.00 ; 100μg $367.00

sp-pme101174 msmp sp1

ECD Proteins

Human MSMP Protein, hFc Tag

SKU:  PME101174     Target:  MSMP     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-PME101168 CXCL8 Fig.1 SDS PAGE 1

ECD Proteins

Human IL8 Protein, His Tag

SKU:  PME101168     Target:  IL8     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101167 vps4 sp1

ECD Proteins

Human VPS4 Protein, His Tag

SKU:  PME101167     Target:  VPS4     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $75.00; 50μg $259.00 ; 100μg $389.00

sp-pme101172 pdgfd sp1

ECD Proteins

Human PDGFD(250-370) Protein, hFc Tag

SKU:  PME101172     Target:  PDGFD     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101166 pdgfd sp1

ECD Proteins

Human PDGFD(19-370) Protein, His Tag

SKU:  PME101166     Target:  PDGFD     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101165 il17f sp1

ECD Proteins

Human IL17F Protein, His Tag

SKU:  PME101165     Target:  IL17F     Tag:  N-6×His Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101164 nrp2 sp1

ECD Proteins

Human NRP2 Protein, His Tag

SKU:  PME101164     Target:  NRP2     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101163 lrg1 sp1

ECD Proteins

Human LRG1 Protein, His Tag

SKU:  PME101163     Target:  LRG1     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101162 agtr1 sp1

ECD Proteins

Human AGTR1 Protein, hFc Tag

SKU:  PME101162     Target:  AGTR1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101160 il10 sp1

ECD Proteins

Human IL10 Protein, His Tag

SKU:  PME101160     Target:  IL10     Tag:  N-6×His Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00