Synthetic Nanodisc: A Polymer-Based Solution for Multipass Transmembrane Proteins

DIMA Biotech’s Synthetic Nanodiscs offer a powerful solution for advancing membrane protein research and drug discovery. Our polymer-based nanodisc platform stabilizes and purifies full-length multi-pass membrane proteins without the use of membrane scaffold proteins (MSPs), resulting in a cleaner system with fewer interferences in downstream assays. We’ve successfully developed over 500 off-the-shelf Nanodisc proteins, covering a wide range of challenging targets such as GPCRs, ion channels, and transporters. Our portfolio includes high-complexity proteins such as the 24-pass transmembrane ion channel protein SCN5A (Cat. No. FLP100726), showcasing our deep expertise in working with difficult membrane proteins. Explore how our Synthetic Nanodiscs enable reliable purification and functional analysis of membrane proteins in their native-like form.

Explore Our Nanodisc Collection!

New: Negative Control – Synthetic Nanodisc

Price: 10 μg $630.00; 50 μg $3080.00; 100 μg $5600.00

SKU: FLP100000

Click here to watch the webinarRevolutionizing Drug Development Synthetic Nanodiscs

Synthetic Nanodisc Flyer Product List Blog ELISA protocol for full length synthetic nanodisc protein Click here to view PeptiNanodisc – ideal for cell-based assays

Case Studies

Human SLC7A11 full length protein-synthetic nanodisc (Cat# FLP100048) 12 pass transmembrane protein

Human SLC7A11-Nanodisc, Flag Tag on SDS-PAGE

Flagged tagged SLC7A11-Nanodisc (FLP100048) can bind anti-Flag mAb and the EC₅₀ is 4.101ng/ml.

The purity of Human SLC7A11-Nanodisc (FLP100048) is greater than 90% as determined by SEC-HPLC.

Human CLDN18.2 full length protein-synthetic nanodisc (Cat# FLP100014) 4 pass transmembrane protein

page-FLP100014 CLDN18.2 Fig.2 SDS PAGE 1

Human CLDN18.2-Nanodisc, Flag Tag on SDS-PAGE

Human CLDN18.2-Nanodisc (0.2μg/per well) binds to anti- CLDN18.2 mAb (Cat. No. BME100075) (EC50 593.6ng/ml).

Human CLDN18.2-Nanodisc can bind Anti-CLDN18.2 antibody (BME100075) with an affinity constant of 1.619 nM as determined in a SPR assay.

Human MDR-1 full length protein-synthetic nanodisc (Cat# FLP100029) 12 pass transmembrane protein

Human MDR-1-Nanodisc, Flag Tag on SDS-PAGE

MDR-1-Nanodisc (FLP100029) can bind anti-Flag monoclonal antibody and the EC50 is 6.883ng/ml.

Human GPRC5D full length protein-synthetic nanodisc (Cat# FLP100011) 7 pass transmembrane protein

Human GPRC5D-Nanodisc, Flag Tag on SDS-PAGE

GPRC5D-Nanodisc (FLP100011) can bind anti-GPRC5D mAb (DME100090) and the EC50 is 32.86ng/ml.

Human CD63 full length protein-synthetic nanodisc (Cat#: FLP100030)

High Activity

ELISA

High Stability

Lyophilization test

Accelerated Test (Lyophilized Powder)

pages-Full length Membrane Proteins High Activity High Stability 6

Synthetic Nanodiscs

Nanodisc Platform

Advantages:
・Full length membrane proteins with high purity
・High solubility in aqueous solutions
・High stability, lyophilized powder stable at room temperature
・Proteins are in a native membrane environment and remain biologically active
・No detergent, useful for cell-based assays
・No MSP backbone proteins

Applications:
・ELISA
・SPR affinity analysis
・Phage display screening
・Immunization
・Protein crystal structure analysis

Nanodiscs Products & Service

Off-the-shelf Nanodisc Proteins

Full Length Transmembrane Proteins

1501 Products

The structure of MSP-Nanodisc (left) and Synthetic Nanodisc (right)

Blog

Synthetic Nanodisc, A Promising Solution for “Undruggable” Membrane Proteins